Adherent cells can be easily assessed for ROS production using this H2DCFDA ROS Assay Kit. This protocol is based upon a working volume of 100uL a test in a 96 well microplate. Protocol: Seed a dark clear-bottomed 96-well microplate with 50,000 cells per well then culture overnight under...
The DCFDA assay protocol is based on the diffusion of DCFDA / H2DCFDA / DCFH-DA / DCFH into the cell. It is then deacetylated by cellular esterases to a non-fluorescent compound, which is later oxidized by ROS into 2’, 7’ –dichlorofluorescein (DCF). DCF is highly fluorescent and ...
DCFDA assay protocol / ROS assay protocol summary (flow cytometry):- collect cells in tubes- stain with DCFDA for 30 min (without washing)- analyze with flow cytometer DCFDA assay protocol / ROS assay protocol summary (fluorescent microscopy):- wash adherent cells with buffer- stain with DCFDA...
This article introduces the detailed operation protocol and attentions for ROS detection using 2′,7′-dichlorodi-hydrofluorescein diacetate (H2DCFDA) fluorescent probe based on flow cytometry and confocal microscope. These methods can be used to detect ROS levels and distribution in model plant ...
Protocol Cell experiment [1, 2]: Cell lines hCECs Preparation Method Telomerase-immortalized human corneal epithelial cells (hCECs) were cultured at 37℃ under 5% CO2 atmosphere in bronchial epithelium growth medium supplemented with 5 mg/mL insulin, 0.5 mg/mL hydrocortisone, a mixture of 50 mg/...
In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. H2DCFDA is dissolved in DMSO to obtain a 10 mM stock solutions and further diluted before use. 2. Cells are incubated with 5 ...
H2DCFDA (DCFH-DA) 是一种可渗透细胞的,用于检测细胞内活性氧 (ROS) 的探针 (Ex/Em=488/525 nm)。
The protocol I have used for ROS detection by this dye is straightforward: 1) Stable cells derived from MDA-MB-231 mammary carcinoma cells were plated in 6 well plates at 3 x 105in full serum media 2) Approx. 24 hours later the cells were treated with 10 uM Hydrogen Peroxide (H...
theprotocol.Cellswerethenanalyzedona fluorescentplatereader.Mean+/-standard deviationisplottedfor4replicatesfromeach condition.TBHPmimicsROSactivityto oxidizeDCFDAtofluorescent DCF. Our Abpromise to you: Quality guaranteed and expert technical support
Incubate the cells according to your normal protocol.2. Treat cells with 10 μM CM-H2DCFDA for 30 min at 37°C in darkness.3. Wash the excess probe.4. Analyze sample on a flow cytometer, fluorescence microscopy, or fluorescence microplate reader. 相关产品:Acetylcysteine | H2DCFDA | ...