Flow-cytometryImmature myeloid cells (ImCCells constantly generate reactive oxygen species (ROS) during aerobic metabolism. The ROS generation plays an important protective and functional role in the immune system. The cell is armed with a powerful antioxidant defense system to combat excessive ...
2.5 Flow cytometry assay Both adherent and non-adherent cells can be assessed for ROS production using this H2DCFDA ROS Assay Kit using flow cytometry. Protocol: Culture cells following established protocols such that around 1.5x104 cells are available per experimental condition. Cells should not...
This article introduces the detailed operation protocol and attentions for ROS detection using 2′,7′-dichlorodi-hydrofluorescein diacetate (H2DCFDA) fluorescent probe based on flow cytometry and confocal microscope. These methods can be used to detect ROS levels and distribution in model plant ...
Notes FlowCytFlowCyt FunctionalStudiesFuncS DCFDA-CellularReactiveOxygenSpeciesDetectionAssayKitimages FlowCytometry-DCFDA-Cellular ReactiveOxygenSpeciesDetection AssayKit(ab113851) ab113851(DCFDA)labeledandunlabeled Jurkatcellsweretreatedwith50µMtert-butyl HydrogenPeroxide(tbHP),thenanalyzedby flowcytometry. ...
DCF-DA has been used as a detector of ROS for a number of years in many applications such as fluorescence microscopy and flow cytometry. This dye is non fluorescent when chemically reduced, but after cellular oxidation and removal of acetate groups by cellular esterases it becomes fluore...
and 0.15 mg/mL BSA. They were then subcultured with 0.25% trypsin-EDTA every 3–4 days prior to use in this study. Incubate the cells with H2DCFDA(DCFH-DA). Then detached cells from the culture wells using 0.25% trypsin-EDTA and washed twice using ice-cold PBS. Flow cytometry measureme...
Determining pollen viability and other physiological parameters is of critical importance for evaluating the reproductive capacity of plants, both for fundamental and applied sciences. Flow cytometry...doi:10.1007/978-1-0716-0672-8_11Nicholas RutleyGad Miller...
Flow cytometry measurements were performed three times for each treatment. . Reaction Conditions H2DCFDA (DCFH-DA) concentration:10 μM; incubate with hCECs at 37℃ for 30 minutes in the dark. Applications H2DCFDA(DCFH-DA) is a redox-sensitive fluorescent, which could be used to measure ...