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DCFH-DA ROS Assay Kit KP-06-003 250-500-1000 test Index Introduction Materials Assay Principle Reagent Preparation Assay Protocol Data Analysis Warranties and Limitation of Liability Pag. 1 Pag. 2 Pag. 3 Pag. 4 Pag. 6 Pag. 9 Pag.10 All chemicals should be handled with care ➢ ➢ ...
Protocol Cell experiment [1, 2]: Cell lines hCECs Preparation Method Telomerase-immortalized human corneal epithelial cells (hCECs) were cultured at 37℃ under 5% CO2 atmosphere in bronchial epithelium growth medium supplemented with 5 mg/mL insulin, 0.5 mg/mL hydrocortisone, a mixture of 50 mg/...
Assay Protocol I. DCF Dye Loading 1. Prepare and mix all reagents thoroughly before use. Each unknown sample should be assayed in duplicate or triplicate. 2. Culture cells in either a clear or black 96-well cell culture plate. Note: If using a black plate, choose an appropriate plate ...
Protocol Cell experiment [1, 2]: Cell lines hCECs Preparation Method Telomerase-immortalized human corneal epithelial cells (hCECs) were cultured at 37℃ under 5% CO2 atmosphere in bronchial epithelium growth medium supplemented with 5 mg/mL insulin, 0.5 mg/mL hydrocortisone, a mixture of 50 mg/...