In this chapter, we describe the dCas9-APEX2 experimental approach for specifically targeting a DNA sequence, enzymatically labeling local proteins with biotin, and quantitatively analyzing the labeled proteome. We also discuss the optimization and extension of this pipeline to facilitate its use in ...
Myers, S.A., Wright, J., Peckner, R.et al.Discovery of proteins associated with a predefined genomic locus via dCas9–APEX-mediated proximity labeling.Nat Methods15, 437–439 (2018). https://doi.org/10.1038/s41592-018-0007-1
pEJS578_DD-dSpyCas9-mCherry-APEX2 临近标记物APEX2 PURPOSE Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors BACKBONE Vector backbone pHAGE-DEST Backbone size w/o insert (bp)6000 Total vector size (bp)12774 Vector type Mammalian Ex...
C-BERST: Defining subnuclear proteomic landscapes at genomic elements with dCas9- APEX2. Nat. Methods (2018). doi:10.1038/s41592-018-0006-2Gao XD, Tu LC, Mir A, et al. C-BERST: defining subnuclear pro- teomic landscapes at genomic elements with dCas9-APEX2. Nat Methods. ...