Mapping proteomic composition at distinct genomic loci in living cells has been a long-standing challenge. Here we report that dCas9–APEX2 biotinylation at genomic elements by restricted spatial tagging (C-BERS
In this chapter, we describe the dCas9-APEX2 experimental approach for specifically targeting a DNA sequence, enzymatically labeling local proteins with biotin, and quantitatively analyzing the labeled proteome. We also discuss the optimization and extension of this pipeline to facilitate its use in ...
Myers, S.A., Wright, J., Peckner, R.et al.Discovery of proteins associated with a predefined genomic locus via dCas9–APEX-mediated proximity labeling.Nat Methods15, 437–439 (2018). https://doi.org/10.1038/s41592-018-0007-1
pEJS578_DD-dSpyCas9-mCherry-APEX2 临近标记物APEX2 PURPOSE Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors BACKBONE Vector backbone pHAGE-DEST Backbone size w/o insert (bp)6000 Total vector size (bp)12774 Vector type Mammalian Ex...
DD-dSpyCas9-mCherry-APEX2(Plasmid #108570) 上海海吉浩格生物科技有限公司 http://hedgehogbio.com测序及更多信息: 点击联系我们QQ:1822235623 微信:1822235623,17031145927 HH-CAS-200 Lenti-iCas9-EGFP-rtTA-Advanced(pinducer20-iCas9+EGFP) HH-CAS-199 Lenti-iCas9-MCS-HA-rtTA-Advanced (pinducer20-i...
Discovery of proteins asso- ciated with a predefined genomic locus via dCas9-APEX-mediated proximity labeling. Nat Methods. 2018;15:437‐439.Myers, S. A. et al. Discovery of proteins associated with a predefined genomic locus via dCas9-APEX-mediated proximity labeling. Nat. Methods ...
Discovery of proteins asso- ciated with a predefined genomic locus via dCas9-APEX-mediated proximity labeling. Nat Methods. 2018;15:437‐439.Myers, S. A. et al. Discovery of proteins associated with a predefined genomic locus via dCas9-APEX-mediated proximity labeling. Nat. Methods ...