The variable numbers of tandem repeats (VNTR) locus D1S80 , located on chromosome 1 (1p35-36), has a repeat unit 16bp in length, and different numbers of these repeat units have been observed for populations of different origins and ethnicity. We used a molecular identification method based...
The D1S80 locus was previously used for forensic analysis due to its small size; however, this locus has recently been used as an associative tool in the elucidation of the chromosome 1p36 deletion syndrome which is one of the most common deletion syndromes located near a chromosomal terminus (...
Nakamura Y, Carlson M, Krapcho V & White R, Isolation and mapping of a polymorphic DNA sequence (pMCT118) on chromosome 1p (D1S80), Nucleic Acids ... M Carlson,Y Nakamura,K Krapcho,... - 《Nucleic Acids Research》 被引量: 324发表: 1987年 Isolation and mapping of a polymorphic DNA ...
利用D1S80座位两侧序列设计的引物(Kasai et al,1990),通过PCR反应,很容易确定特定个体的D1S80等位基因构成,纯合体只有一条DNA带,而杂合体有两条不同的DNA带。 1.将小组的电泳结果拍照,并把照片贴在实验报告上,对照片进行必要的说明,例如,相对分子质量的标记各片段的大小(bp)。 (见附图) 2.根据电泳结果,...
· Eiji Kajii A Japanese propositus with D-- phenotype characterized by the deletion of both the RHCE gene and D1S80 locus situated in chromosome 1p and the existence of a new CE-D-CE hybrid gene Received: September 21, 1999 / Accepted: January 5, 2000 Abstract In a family study of a...
列如,人类1号染色体上的VNTR D1S80,核心序列由16个核苷酸组成,拷贝数在14～41个之间,已知29种不同的等位基因。 1984年Jefferys等人首次将分离的人源小卫星DNA用作基因探针,同人类核DNA的酶切片段杂交,产生了由10多条带组成的杂交图谱,不同个体杂交图谱上带的位置就像指纹一样因人而异,因而Jefferys等人称之为...
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