D1S80,P33.6VNTR位点多态性基因扩增法医学用PCR技术,小型垂直板聚丙烯酰胺凝胶电泳及银染显色法,分析血痕,精斑,混合斑中的D1S80,P33.6VNTR位点的扩增片段长度多态性(Amp-FLP)获得了满意的结果,将该方法运用于案件的鉴定,在破案中发挥了重要的作用.景强昆明医学院法医学院程宝文云南省公安厅vip昆明医科大学学报...
PCR-amplification and detection of the human D1S80 VNTR locus A series of experiments has been performed to evaluate amplification and typing of the D1S80 VNTR locus. The validation study that has been carried out sho... AD Kloosterman,BP Daselaar - 《International Journal of Legal Medicine》...
DNA from the locus D1S80 was amplified by polymerase chain reaction (PCR) and analyzed by electrophoresis in vertical polyacrylamide gels followed by silverstaining. DNA samples from 119 unrelated Danes and 97 mother/child pairs were examined. The amplified fragment length polymorphism (AMP-FLP) ...
Budowle B, Chakraborty R, Giusti AM, Eisenberg AJ, Allen RC (1991) Analysis of the VNTR Locus D1S80 by the PCR Followed by High-Resolution PAGE. Am J Hum Genet, 48: 137–144 Elder JK, Southern EM (1983) Measurement of DNA Length by Gel Electrophoresis II: Comparison of Methods for...
产生DNA指纹图谱的过程叫做DNA指纹分析,目前包括PCR、RFLP(限制性内切酶酶切片段长度多态性)和RAPD(随机扩增多态性DNA)等方法。 DNA指纹图谱的基本特点: (1)多位点性:基因组中某些位点的小卫星重复单位含有相同或相似的核心序列。在一定的杂交条件下,一个小卫星探针可以同时与十几个甚至几十个小卫星位点上的等位...
The semi-nested PCR has been applied to the amplification of D1S80 nucleic acid sequences. For amplification of the locus D1S80, a pair of oligonucleotide primers have been used widely as described by Kasai et al. We have designed another set of primers for semi-nested PCR. This method ...
The amplification of the D1S80 locus, a variable number of tandem repeats (VNTR) locus, by the polymerase chain reaction (PCR), (Saiki et al, 1985) is an effective tool to forensic analysis (Budowle et al, 1995).doi:10.1007/978-3-642-80029-0_190Santos, S. M....
1993. PCR- amplification and detection of the human D1S80 VNTR locus. Amplification conditions, population genetics and application in forensic analysis. Int... AD Kloosterman,BP Daselaar - 《International Journal of Legal Medicine》 被引量: 363发表: 1993年 Fractal Analysis of Coal Pore Structure...
利用D1S80座位两侧序列设计的引物(Kasai et al,1990),通过PCR反应,很容易确定特定个体的D1S80等位基因构成,纯合体只有一条DNA带,而杂合体有两条不同的DNA带。 1.将小组的电泳结果拍照,并把照片贴在实验报告上,对照片进行必要的说明,例如,相对分子质量的标记各片段的大小(bp)。 (见附图) 2.根据电泳结果,...
The total range of repeat lengths (approx. 0.2 to 1kb) and the relatively small number of repeats (generally 14 to >40) within the unit make this locus suitable for PCR analysis [15, 20] (Figure 1). The observed heterozygosity has been reported as high as 90.5% and as low as 24% ...