BD Biosciences Protocol : Cytokine Flow Cytometry of PBMCs in 96 Well Plates DescriptionReagents, ActivationMicroscopy, Electron
- 1996 () Citation Context ...alyzed by flow cytometry using a FACScan (BD Biosciences). The acquired data were analyzed with CellQuest software (BD ... E Bottius,L Benmohamed,K Brahimi,... - 《Journal of Immunology》 被引量: 153发表: 1996年 Detection of intracellular antigens in porc...
RNA flow cytometryUnderstanding how immune cells respond to external stimuli such as pathogens or drugs is a key component of biomedical research. Critical to the immune response are the expression of cell-surface receptors and the secretion of cytokines, which are tightly regulated by gene ...
Results We have developed a protocol for whole blood stimulation and processing in deep-well 24- or 96-well plates, and fresh or cryopreserved peripheral blood mononuclear cell (PBMC) stimulation and processing in conventional 96-well round-bottom plates. Samples from both HIV-1-seronegative and ...
3. b, Intracellular expression of TCF-1 in the indicated CART-123 + T cell subsets, measured by flow cytometry. MFI displayed to the right. Representative of two experiments with different primary AML and CART-123 donors complements distinct donors shown in Fig. 3. See also, ...
Flow cytometry Flow cytometry was carried out on single-cell suspensions of whole lung, spleen, and liver tissues. A standard protocol was used to prepare single-cell suspensions: (i) lungs and liver were minced and then digested with 0.2 mg/ml collagenase type IV (Worthington Biochemical ...
Learning Resources Product Selection Primary Antibody Selector Tool Secondary Antibody Selector Tool Fluorescence SpectraViewer Flow Cytometry Panel Builder Related Product Categories Antibodies Flow Cytometry Cell Analysis Quick protocol guidelines...
BProtocol: Staining for Flow Cytometry The procedure for activating cells is given on page 429 (see notes 2 and 3). editor: page of activation procedure) If cell surface staining is performed, start with step 1; forintracellular cytokine stainingwithout cell surface staining, start with step 7...
The human biological samples were sourced ethically, and their research use was in accord with the terms of the informed consents under an IRB/EC approved protocol within each individual laboratory’s organization. Appendix A. Supplementary material The following are the Supplementary data to this art...
and did not meet euthanasia criteria were harvested on day 60 at the conclusion of the study. Spleens and lungs were weighed to assess the potential toxicity of treatment. Spleens were subjected to immunophenotyping by flow cytometry to quantify expansion of CD8+ T cells using the ...