Flow cytometryAntibodyIntracellular cytokine detectionAnalysis of intracellular cytokines is extremely important in the clinical treatment of numerous diseases. Flow cytometry (FCM) is a highly effective technique that detects intracellular cytokines using specific fluorescence-labeled antibodies. The common steps...
Intracellular cytokine detection by flow cytometry in pigs: Fixation, permeabilization and cell surface staining Intracellular flow cytometry is a method of cytokine detection that allows simultaneous detection of intracellular cytokines and cell surface markers. This... P Zelnickova,M Faldyna,H Stepanova...
Either all or the terminal portion of the incubation is done in the presence of a secretion inhibitor such as brefeldin A, which allows the detection of cytokines intracellularly The short-term incubation, often 6 h, avoids the confounding effects of cell proliferation and/or apoptosis and thus ...
We defined a flow cytometry method that combines the conventional measurement of T cell surface proteins (CD45, CD3, CD4, CD8) and intracellular cytokines (IL-2, INF-纬) with fluorescent in situ hybridization and branched DNA technology for amplification and detection of IL-2 and INF-纬 mRNA...
Comparison of the ELISPOT and cytokine flow cytometry assays for the enumeration of antigen-specific T cells The enumeration of antigen-specific T cell responses has been greatly facilitated in recent years by the development of methods based on the detection of c... AC Karlsson,JN Martin,SR You...
To enable detection of expression signatures specific to individual cells we developed PLAYR (ProximityLigationAssayforRNA). PLAYR enables highly multiplexed quantification of transcripts in single cells by flow- and mass-cytometry and is ... AP Frei,FA Bava,ER Zunder,... - 《Nature Methods》 ...
Detection of antigen-specific T cells by cytokine flow cytometry: the use of whole blood may underestimate frequencies Antigen-specific T cells may be detected and enumerated by short-term ex vivo antigen-specific stimulation followed by cytokine flow cytometry. Most fre... Bodo,Hoffmeister,...
The aim of this report is to outline the basic proto-cols and applications for the detection of intracellular cytokines by flow cytometry, in the context of disease monitoring. 展开 关键词: antibody targeting cancer ADEPT CEA sFv fusion protein ...
Cytometry 46:33–40 - Bueno, Almeida, et al. - 2001 () Citation Context ...andards. Analysis of intracellular cytokine production in DCs, monocytes, and T cells. Intracellular cytokine detection was performed by flow cytometry as previously described with some modifications =-=(1, 7...
Intracellular cytokine detection by flow cytometry has emerged as the premier technique for studying cytokine production at the single-cell level. Multipar... B Foster,C Prussin - John Wiley & Sons, Inc. 被引量: 497发表: 2002年 Cytokine Profiles for Peripheral Blood Lymphocytes from Patients wi...