Neupert. 1983. Biogenesis of cytochrome c in Neurospora crassa. Methods Enzymol. 97:261-274.Korb H, Neupert W. 1978. Biogenesis of cytochrome c in Neurospora crassa. Synthesis of apocytochrome c, transfer to mitochondria and conversion to holocytochrome c. Eur. J. Biochem. 91:609-20...
Cytochromes of c-type contain covalently attached hemes that are formed via thioether bonds between the vinyls of heme b and cysteines within C 1XXC 2H motifs of apocytochromes. In diverse organisms this post-translational modification relies on membrane-associated specific biogenesis proteins, referred...
Cytochrome c biogenesis is the covalent ligation of heme b to an apocyt c. • Ccm is an intricate post-translational protein modification process. • Ccm-System I involves nine membrane-bound proteins working together. • The Ccm proteins are proposed to form a large multi subunit supercomp...
A mutant cell defective in such a system lacks cytochrome c specifically and can synthesize all other kinds of heme proteins. The case of cytochrome c demonstrates clearly that there exist sophisticated and dedicated machineries for heme protein biogenesis in cells. Among the still unresolved ...
It is generally agreed that cytochrome c biogenesis requires that the apocytochrome and heme be transported separately to their site of function and assembly. In bacteria, this is outside the cytoplasmic membrane, whereby the apocytochromes c use sec-dependent signals for their translocation. Two di...
(2012) Adaptation of respiratory chain biogenesis to cytochrome c oxidase defi- ciency caused by SURF1 gene mutations. Biochim. Biophys. Acta 1822, 1114-1124.N. Kovarova, A. Cizkova Vrbacka, P. Pecina, V. Stranecky, E. Pronicka, S. Kmoch, J. Houstek, Adaptation of respiratory ...
Strikingly, PHCP with a covalently bound heme was heterologously synthesized in the periplasm of Escherichia coli strains deficient in the DsbD protein, a component of the System I cytochrome c biogenesis machinery. The heterologous synthesis of PHCP by aerobically growing E. coli also occurred ...
S9a, b, ectopic expression of KRasG12V had little impact on mitochondrial biogenesis, as evidenced by the comparable ratio of mitochondrial DNA to nuclear DNA and citrate synthase levels. Additionally, ectopic expression of KRasG12V had little effect on MT-CO2 mRNA stability (Supplementary Fig. ...
Highlights ► Structure-function, regulation and biogenesis of cbb3-type Cox are reviewed. ► A multistep assembly pathway of the subunits of this membrane enzyme is discussed. ► A model for formation of the heme b3-CuB center of this enzyme is proposed.Previous...
SURF1, encoding a factor involved in the biogenesis of cytochrome c oxidase, is mutated in Leigh syndrome. Leigh Syndrome (LS) is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions that is common... Z Zhu,J Yao,T Johns,... - ...