CYP2D6*10 (rs1065852)位点基因分型Taqman探针结果如图1。 图1 CYP2D6*10(c.100 C>T)位点基因分型Taqman探针结果 Fig.1 Result of CYP2D6*10 (c.100 C>T) genotyping by Taqman PCR. A: Amplification plot of CYP2D6*10 (c.100 C>T) CC genotype; B: Amplification plot of CYP2D6*10 (c.100...
The CYP2D6*10 alleles (rs1065852) were detected. Eleven patients carried the CYP2D6*10 C/C genotype, 42 patients carried the C/T genotype, and 17 patients carried the T/T genotype. Four patients (3 C/T and 1 T/T) took iloperi- done at a dose of 12 mg/d, 12 patients (2 C/...
本发明公开了一种焦磷酸测序法检测他莫昔芬个体化用药基因多态性试剂盒及方法.具体是指CYP2D6*10(rs1065852)和(SULT1A1*2)(rs9282861)单核苷酸多态性.试剂盒包含如SEQIDNO.3-8所示的引物.本发明的试剂盒,可以实现准确,快捷,高通量CYP2D6*10(rs1065852)和SU... 周宏灏 被引量: 0发表: 0年 Clinical im...
本发明的试剂盒利用高度特异的引物对CYP2D6(rs1065852)进行精准的靶向扩增,结合Taqman探针高特异性、快速简便的优点实现CYP2D6(rs1065852)的基因分型。权利要求书1页说明书4页序列表1页附图2页CN112695097B2022.08.19CN112695097B1.一种用于区分假基因CYP2D7P和CYP2D8P从而扩增和分型CYP2D6*10遗传多态性的引物...
Com- pared to the two alleles of 188C and 4268G in CYP2D6*1 (wild type), CYP2D6*10 has two single nucleotide polymorphisms (SNP) 188T (rs1065852, Pro34Ser) and 4268C (rs1135840, Ser- 486Thr) (http://www.cypalleles.ki.se/cyp2d6.htm), and the frequencies are 38%–70% in ...
7.针对上述现有技术的不足,本发明提供了一种用以区分假基因cyp2d7p和cyp2d8p从而精准扩增和分型cyp2d6*10遗传多态性的检测试剂盒,利用高度特异的引物对cyp2d6(rs1065852)进行精准的靶向扩增,结合taqman探针高特异性、快速简便的优点实现cyp2d6(rs1065852)的基因分型。
一种区分CYP2D7P和CYP2D8P的CYP2D6*10遗传多态性检测试剂盒.pdf,本发明公开了一种区分CYP2D7P和CYP2D8P的CYP2D6*10遗传多态性检测试剂盒,属于分子医学技术领域。本发明的试剂盒利用高度特异的引物对CYP2D6(rs1065852)进行精准的靶向扩增,结合Taqman探针高特异性、快速简
应用TaqMan探针技术检测两组孕妇CYP2C9基因rs1057910位点和CYP2D6基因rs1065852位点基因型分布及等位基因频率,研究CYP2D6和CYP2C9基因多态性与重度子痫前期母婴结局的相关性.结果 研究组患者CYP2D6多态性基因型频率明显大于对照组孕妇,CYP2D6基因rs28371725和CYP2C9基因rs4918758位点基因型和等位基因频率显著高于对照组...