本发明公开的一种检测CYP2C9基因多态性的ARMS引物,针对CYPC2C9*2(rs1799853,C430T,Arg144Cys)和CYP2C9*3(rs1057910,A1075C,Ile359Leu)每个SNP的序列改变而设计的特异性ARMS引物和共用引物,特异性ARMS引物于3'末端第二位或三位引入错配碱基(下划线标记碱基),以此扩大特异性引物3'末端碱基与DNA模版上SNP位点...
CYP2C9*2 (rs1799853) was amplified using PCR primers Fw: 5′-NNGTATTTTGGCCTGAAACCCATA-3′ and Re: 5′-BIOTIN-CACCCTTGGTTTTTCTCAACTC-3′ (Biomers), which yield a 455-bp nucleotide sequence. PCR was performed in 25-μl reactions using 25 ng DNA, 0.2 μ M of each primer, ...
cyp2c9参与抗凝血药、抗惊厥药、降糖药、非甾体类解热镇痛抗炎药、抗高血压药以及利尿药等多种药物的羟化代谢,其中华法林、甲苯磺丁脲和苯妥因均为治疗指数较窄的药物。cyp2c9活性变化可导致这些药物体内浓度出现较大变化,甚至导致严重药物不良反应的发生。cypc2c9*2(rs1799853,c430t,arg144cys)和cyp2c9*...
CYP2C9 gene contains several variants, but two of the most prevalent, investigated, and influential of them are rs1799853 and rs1057910, also known as CYP2C9*2 and CYP2C9*3 polymorphisms, respectively. CYP2C9*2 results in an amino acid substitution from Arg to Cys as a consequence ...
Besides this, other functional variants rs1799853 (p.Arg144Cys) and rs72558189 (p.Arg335Trp) were observed in 10 and six samples of NGS data repository, respectively. These var iants are associated with CYP2C9*2 and *14 haplotypes (Table 3). In total, eight rare and putative ...
(CYP2C9*3) and rs1799853(CYP2C9*2) were significantly associated with decreased metabolism of SUs inhealthy volunteers[60]. TheCYP2C9*3,Ile359Leu and Arg144Cys variants ofCYP2C9gene were found to enhance risk of hypoglycemia or cause a major reduction inrenal clearanceof glibenclamide ...
摘要 本发明提供了一种采用分子信标探针检测人类CYP2C9基因多态性试剂盒,包含以下核酸序列:扩增CYP2C9基因rs1799853、rs1057910多态性位点的引物对和检测用的探针。本发明还公开了上述引物和探针在检测扩增CYP2C9基因多态性位点的应用。本发明可以在一个PCR反应中快速检测CYP2C9基因rs1799853、rs1057910位点多态...
All patients were genotyped for CYP2C9 rs1799853 and rs1057910 (*2 and *3 allele, respectively), KCNJ11 rs5219 and rs5215, and ABCC8 rs757110. Results:The average duration of diabetes in the study group was 10.6±7.1 years. Most of the patients achieved relatively good blood glucose ...
The most widely studied variants are CYP2C9*2 (p.R144C; rs1799853) and CYP2C9*3 (p. I359L; rs1057910), which seem to decrease the metabolic activity of this enzyme, mainly CYP2C9*3. This reduction in metabolic activity was confirmed in different in vivo studies, using drugs such...
rs1799853rs1057910CYP2C19/CYP2C9 haplotypesVenezuelaBackground: Polymorphisms with decreased enzyme activity of their gene products have been reported in region CYP2C with population variations in haplotype structure.doi:10.1080/03014460.2016.1192218Flores-Gutiérrez, Sara...