加一抗(JunB和cyclin D1一抗购自美国Santa Cruz公司,FosB一抗购自美国Cell Signaling公司,均为1︰1 000稀释;内参照β-actin,1︰5 000稀释,美国Bioworld公司)4 ℃过夜,加二抗后室温避光孵育1 h,在Clinx ChemiScope化学发光成像系统进行增强...
6.2Cyclin D Cyclin Dis a keycell cycle protein, which is considered to be a short-livednuclear proteinand regulates the cell cycle(84).Cyclin D1is regarded as a marker for aggressivemetastasisinPCa(85). InAD, an up-regulation of cyclin D associated CDK (Cyclin dependent kinase) has been ...
A recent study reveals that cyclin D1 acts to promote cell migration by inhibiting Rho/ROCK signaling and expression of thrombospondin-1 (TSP-1), an extracellular matrix protein that regulates cell migration in many settings including cancer. Given the frequent overexpression of cyclin D1 in ...
抗CyclinD1、E2F-1 抗体由Santa Cruz Biotechnolo- gy 公司提供,抗VEGF、pRb、MMP-2 和MMP-9 抗体由 Cell Signaling Technology 公司提供。Cy- clinD1 反义寡核苷酸由北京赛百盛生物科技有限公司合成。T4 DNA 连接酶、EcoRI 内切酶、HindIII 内切酶均购自Fermentas 公司,胶回收试剂盒购自大连宝生物生物技术有限...
Background: Cyclin D1 is an important protein for cell cycle progression; however, functions independent of the cell cycle have been described in the liver. Cyclin D1 is also involved in DNA repair, is overexpressed in many cancers, and functions as a proto-oncogene. The lesser-known roles ...
Cell migration is essential for developmental morphogenesis, tissue repair, and tumor metastasis. A recent study reveals that cyclin D1 acts to promote cell migration by inhibiting Rho/ROCK signaling and expression of thrombospondin-1 (TSP-1), an extracellular matrix protein that regulates cell migrat...
reduction of BrdU+c cells in DG and SVZ zone was found in cyclin D 1 gene knockout (cyclin D 1-/- ) mice compared with cyclin D 1+/+ and Cyclin D 1+/- mice.The results indicate that cyclin D 1 may have a critical role in differentiation and proliferation of neural stem cell. ...
1、CyclinD1和ras过表达在胃粘膜细胞癌变中的协同作用摘要:目的探讨cyclin D1和ras在胃癌中的相互关系。方法运用免疫组化方法检测cyclin D1和ras在胃癌中的表达。结果cyclin D1和ras在胃癌中的阳性表达率分别为59.2%和57.1%,二者在胃癌中的表达显著相关(P0.05)。cyclin D1阳性表达率与肿瘤大小和组织学类型显著...
结论:HMGB1能激活STAT1信号转导通路,并上调cyclinD1基因表达,这 可能与HMGBI促进滑膜细胞的增殖有关。 [关键词] 高迁移率族蛋白质1;滑膜细胞;信号转导及转录活化因子;细胞因子信号转导抑制蛋白;细胞 周期蛋白D1 [中图分类号] R361 .3 [文献标识码] A doi:10:3969/j.issn.1000—4718.2010.06.032 Efectof...
Glycogen synthase kinase-3β regulates cyclin D1 proteolysis and subcellular localization The activities of cyclin D-dependent kinases serve to integrate extracellular signaling during Gphase with the cell-cycle engine that regulates DNA replica... JA Diehl,M Cheng,MF Roussel,... - 《Genes & Develo...