Western Blotting 1:1000 Immunoprecipitation 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. Protocol Specificity / Sensitivity Cyclin D1 Antibody detects endogenous levels of cyclin D1....
加一抗(JunB和cyclin D1一抗购自美国Santa Cruz公司,FosB一抗购自美国Cell Signaling公司,均为1︰1 000稀释;内参照β-actin,1︰5 000稀释,美国Bioworld公司)4 ℃过夜,加二抗后室温避光孵育1 h,在Clinx ChemiScope化学发光成像系统进行增强...
( Cell Signaling ) 、STAT1、 STAT3 多克隆抗体 (Santa Cruz) 、SOCS1 和 SOCS3 多 克隆抗体 (Santa) ,小 鼠抗大鼠 cyclin D1 单克隆抗体 ( NeoMarkers) 。DMEM 培养基和胰蛋 白酶 ( Gibco) 、 胎牛血清(北京圣马元亨生物科技公司 ) ,Tri zol(Sig. ma) ,RNasin、Taq DNA polymerase ( Pro...
Cell CycleLi Z, Wang C, Prendergast G, Pestell RG. Cyclin D1 functions in cell migration. Cell Cycle. 2006;5:2440–2.Li Z, Wang C, Prendergast GC, Pestell RG. Cyclin D1 functions in cell migration. Cell Cycle. 2006; 5 :2440–2442. doi: 10.4161/cc.5.21.3428. [ Cross Ref ]...
6.2Cyclin D Cyclin Dis a keycell cycle protein, which is considered to be a short-livednuclear proteinand regulates the cell cycle(84).Cyclin D1is regarded as a marker for aggressivemetastasisinPCa(85). InAD, an up-regulation of cyclin D associated CDK (Cyclin dependent kinase) has been ...
Conclusion URG4, which is high-expressed in osteosarcoma, promotes cell cycle progression via GSK3β/β-catenin/cyclin D1 signaling pathway and may be a novel biomarker and potential target for the treatment of osteosarcoma.Introduction Osteosarcoma is one of the most common malignant bone tumors ...
Cyclin D1 controls development of cerebellar granule cell progenitors through phosphorylation and stabilization of ATOH1 |核心内容: 在发育过程中,神经祖细胞处于增殖和未成熟状态; 然而,协同控制这两种状态的分子机制仍然难以捉摸。 ...
reduction of BrdU+c cells in DG and SVZ zone was found in cyclin D 1 gene knockout (cyclin D 1-/- ) mice compared with cyclin D 1+/+ and Cyclin D 1+/- mice.The results indicate that cyclin D 1 may have a critical role in differentiation and proliferation of neural stem cell. ...
Cyclin D1 controls development of cerebellar granule cell progenitors through phosphorylation and stabilization of ATOH1 |核心内容: 在发育过程中,神经祖细胞处于增殖和未成熟状态; 然而,协同控制这两种状态的分子机制仍然难以捉摸。 在这里,我们报道了细胞周期蛋白 D1(CCND1)直接调节小脑颗粒细胞祖细胞(GCPs)的增殖...
Cyclin D1 controls development of cerebellar granule cell progenitors through phosphorylation and stabilization of ATOH1 |核心内容: 在发育过程中,神经祖细胞处于增殖和未成熟状态; 然而,协同控制这两种状态的分子机制仍然难以捉摸。 在这里,我们报道了细胞周期蛋白 D1(CCND1)直接调节小脑颗粒细胞祖细胞(GCPs)的增殖...