6、为了进一步验证cxcr3抑制剂(amg487)是否能够逆转抑制免疫检查点后诱导的心肌肥厚及心衰,将c57bl/6为背景的t细胞特异性敲除pd-1(lck-cre pd-1fl/fl )小鼠及同窝未敲除pd-1(pd-1fl/fl)的对照小鼠均进行tac手术,术后即开始每日腹腔注射amg487的溶剂(98% v/v玉米油+2% v/v dmso,不含有amg487)
外周CD45+流式染色实验进一步验证肺脏中这群细胞属于lungs-BRMs。随后,作者使用Tfh缺失的小鼠(Bcl6fl/flCd4cre/+)以及Tfh阻断方法(anti-CD40L),均证实lung-BRMs的分化需要Tfh辅助,且这种辅助发生在感染的早期3周内。 紧接着,作者检...
47 Only in the setting of autoimmunity of the kidney (crescentic glomerulonephritis), using Foxp3-driven CRE-specific deletion of T-betfl/fl or Cxcr3fl/fl alleles, was it definitively shown that CXCR3 alone was required for Treg cell recruitment to the kidney to reduce the severity of ...
通过在Cxcr3基因目的exon 2两侧分别插入loxP位点,构建Cxcr3-Flox小鼠。该flox小鼠可与组织特异性Cre工具鼠交配,获得在特定细胞类型或组织中敲除Cxcr3基因的小鼠模型。 *使用本品系发表的文献需注明: Cxcr3-Flox mice (Cat. NO. NM-CKO-2117369) were purchased from Shanghai Model Organisms Center, Inc.. 相...
However, CD8αCre-ALK5flox/flox (ALK5ΔCD8) mice reject tumors in high proportions, dependent on CD8+ T cells. ALK5ΔCD8 mice have more tumor-infiltrating effector CD8+ T cells, with more cytotoxic capacity. ALK5-deficient CD8+ T cells exhibit increased CXCR3 expression and enhanced ...
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in cre ase d l14】 . O ur rece nt stu d ie s do cum e nte d t ha t C X C R 3 is inv olv e d in acu te pu lmon ary in fl a m m a tion ind uce d by short — term ex posu re of C S throu g h re cru itm e nt of C D 8 + T cells in to lung ...
通过在Cxcr3基因目的exon 2两侧分别插入loxP位点,构建Cxcr3-Flox小鼠。该flox小鼠可与组织特异性Cre工具鼠交配,获得在特定细胞类型或组织中敲除Cxcr3基因的小鼠模型。 *使用本品系发表的文献需注明: Cxcr3-Flox mice (Cat. NO. NM-CKO-2117369) were purchased from Shanghai Model Organisms Center, Inc.. ...
Crescioli, C.; Corinaldesi, C.; Riccieri, V.; Raparelli, V.; Vasile, M.; del Galdo, F.; Valesini, G.; Lenzi, A.; Basili, S.; Antinozzi, C. Association of circulating CXCL10 and CXCL11 with systemic sclerosis. Ann. Rheum. Dis. 2018, 77, 1845–1846. [Google Scholar] [...
47 Only in the setting of autoimmunity of the kidney (crescentic glomerulonephritis), using Foxp3-driven CRE-specific deletion of T-betfl/fl or Cxcr3fl/fl alleles, was it definitively shown that CXCR3 alone was required for Treg cell recruitment to the kidney to reduce the severity of ...