*使用本品系发表的文献需注明: Cxcr3-KO mice (Cat. NO. NM-KO-190071) were purchased from Shanghai Model Organisms Center, Inc.. 相关品系 Cxcr3-Flox 品系名:C57BL/6Smoc-Cxcr3em1(flox)Smoc 品系状态:活体 | 目录号:NM-CKO-2117369 hCXCR3 品系名:C5
To rule out the possibility that DT-mediated Treg ablation impacted tumor growth rather than CXCR3 deficiency in Treg cells, we evaluated tumor growth in mice where CXCR3 was left intact on Treg cells. Depleting approximately half of the Treg cells with DT, while retaining CXCR3 in the ...
BMDMs, from either LysMCre or p65fl/fl/LysMCre mice, were seeded into 6-well plates, let rest for 24 hours, and then cancer cells were seeded onto the transwell insert. Cells shared the growth medium for 24 hours before collection and subsequent RNA isolation, cDNA synthesis, and qPCR ...
Materials and methods Animals KrasLSLG12D/+;Trp53LSLR172H/+;p48Cre (KPC) mice pre- viously speed-backcrossed to > 99.6% genetic similarity to C57BL/6 J mice were used [7]. 6- to 12-wk-old female 13 1 474 Cancer Immunology, Immunotherapy (2023) 72:1461–1478 and male ...
Methods:CXCR3knockout(CXCR3-/一)micewereused。Differencesinairspaceenlargement,mRNAexpressionofmatrixmetaIloprotei— nases(MMPs),transforminggrowthfactor(TGF)131,CXCL10inlunghomogenates,andCXCL10contentinbronchoalveolarlavaIge(BAL) fluidsandhomogenateswerecomparedbetweenCXCR3-/一miceandwild甘pe(WT)micethree...
通过在Cxcr3基因目的exon 2两侧分别插入loxP位点,构建Cxcr3-Flox小鼠。该flox小鼠可与组织特异性Cre工具鼠交配,获得在特定细胞类型或组织中敲除Cxcr3基因的小鼠模型。 *使用本品系发表的文献需注明: Cxcr3-Flox mice (Cat. NO. NM-CKO-2117369) were purchased from Shanghai Model Organisms Center, Inc.. ...
Using preclinical colorectal cancer models in cell type-conditional TGFβ receptor I (ALK5) knockout mice, we interrogate this mechanism. Tumor growth delay and radiation response are unchanged in animals with Treg or macrophage-specific ALK5 deletion. However, CD8αCre-ALK5flox/flox (ALK5ΔCD8...
Clec7a-/- mice (Saijo et al., 2007) were provided by Dr. Shinobu Saijo (University of Tokyo). Card9-/- mice (Hsu et al., 2007) were provided by Dr. Xin Lin (Tsinghua University). MRP8-Cre-IRES/GFP and ROSA26 iDTR mice were crossed to generate ROSA26-iDTRMrp8-Cre mice. C57BL...
通过在Cxcr3基因目的exon 2两侧分别插入loxP位点,构建Cxcr3-Flox小鼠。该flox小鼠可与组织特异性Cre工具鼠交配,获得在特定细胞类型或组织中敲除Cxcr3基因的小鼠模型。 *使用本品系发表的文献需注明: Cxcr3-Flox mice (Cat. NO. NM-CKO-2117369) were purchased from Shanghai Model Organisms Center, Inc.. 相...
通过在Cxcr3基因目的exon 2两侧分别插入loxP位点,构建Cxcr3-Flox小鼠。该flox小鼠可与组织特异性Cre工具鼠交配,获得在特定细胞类型或组织中敲除Cxcr3基因的小鼠模型。 *使用本品系发表的文献需注明: Cxcr3-Flox mice (Cat. NO. NM-CKO-2117369) were purchased from Shanghai Model Organisms Center, Inc.. 相...