HumanMacrophageMonocytePhagocytesPolarizationThe studies presented in this thesis demonstrate essential roles for histone deacetylase (HDAC) activity in regulating inflammatory responses of myeloid and stromal cells in rheumatoid arthritis (RA), and suggest that therapeutic targeting of HDACs with small ...
The liver is composed of different cell populations. Interactions of different cell populations can be investigated by a newly established indirect co-culture system consisting of immortalised primary human hepatocytes and human monocyte derived macrophages (MDMs). Using the time-dependent cytokine secretion...
The liver is composed of different cell populations. Interactions of different cell populations can be investigated by a newly established indirect co-culture system consisting of immortalised primary human hepatocytes and human monocyte derived macrophages (MDMs). Using the time-dependent cytokine secretion...
tuberculosis at an early stage of infection of the host lung, many of the cell culture models have focused on the use of murine or human monocyte-derived macrophages or dendritic cells (de Chastellier, 2009). The use of cell cultures enables researchers to isolate the bacterial processes, ...
PCC-derived AREG promotes CCL5 secretion in macrophages through the Hippo-YAP pathway. By targeting the feedback loop, mithramycin improves the outcome of gemcitabine treatment in PAAD. The results from the PDO model were corroborated with cell lines, mouse models, and clinical data. Conclusions ...
Human intestinal stem cell-derived enteroid monolayers co-cultured with human monocyte-derived macrophages were used to evaluate barrier function, cytokine secretion, and protein expression under basal conditions and following bacterial infection. Macrophages enhanced barrier function and maturity of enteroid...
(PRP); and recently, autologous patient serum containing activated monocyte/macrophages (a mixed population termed as MΦs) [7] that are injected with short- to medium-term analgesic effects. OA is managed with these symptom-modifying approaches until surgical joint replacement can be performed—...
To further use the model, we performed in vitro slice contusion injury and studied allogeneic neural cell therapy by grafting human first trimester-derived neural stem/progenitor cells (hfNPCs), to lesioned or control slices. We conclude that human spinal cord tissue slice culture is a suitable ...
The relationship between in vitro alterations of monocyte morphology and biochemistry, as seen during culture, and true differentiation in vivo of blood monocytes to tissue macrophages, however, is not known. Besides study of the process of differentiation, there are numerous other aspects of the ...
Alveolar epithelial cells (NCI-H441 cells) and monocyte-derived macrophages were cocultured within the upper chamber of the chip interfacing an air phase, while human umbilical vein endothelial cells (HUVECs) were cultured within the lower chamber, perfused with culture medium. A stable air–...