Nonalcoholic fatty liver disease (NAFLD) is the most common liver disorder with high morbidity and mortality. The current study aims to explore the role of Cullin-associated and neddylation-dissociated protein 1 (CAND1) in the development of NAFLD and the underlying mechanisms. CAND1 is reduced ...
Cullin 5 (CUL5) (C-term) rabbit polyclonal antibody, Purified
The nomenclature for CRLs is only well established for CUL1-based SCF complexes. The naming of other CRL complexes is not settled, with various competing acronyms. For this review, we will refer to non-SCF CRL complexes by using the acronym "CRL" forcullinRING ubiquitinligase followed by the ...
The asterisk denotes a crossreactivity of the anti-Cul1p antibody. disturbed in the presence of stable amounts of core components and FBPs, we developed a metabolic 15N pulse-labelling assay that allowed us to assess CRL1 complex dynamics. Wild-type and Dknd1 cells were maintained in 14N ...
Similar results were obtained with Cln2p/Cdc28pHA/Cks1p complexes purified on an anti-HA monoclonal antibody matrix (data not shown). The availability of purified proteins allowed us to test whether this set of components was sufficient to direct ubiquitination of MBP-Sic1pMH6. Since the ...
Unfortunately, only available antibody is for Bim. We, therefore, performed the FACS analysis, as described53, to quantify the levels of Bim, and confirmed that Bim is indeed accumulated up to three-fold in Rbx1-depleted Treg cells (Fig. 6a, b). It is noting worthy that our single cell...
we intranasally treated the influenza-induced asthma exacerbations LysMCreCul5fl/flmice with an IFN-β neutralizing antibody (anti-IFN-β Ab) (Fig.3d), which reaggravated mucus deposition, tissue destruction, cell infiltration, and collagen accumulation (Fig.3eand Supplementary Fig.6f). The re-...
Control: n = 8, palbociclib: n = 10, PD-1 antibody: n = 9, palbociclib and anti-PD-1 antibody: n = 8. k, A working model of how PD-L1 protein stability is regulated by the cyclin D–CDK4–SPOP–FZR1 signalling pathway. Cyclin D–CDK4 negatively regulates PD-...
The β-tubulin IIA antibody used in our experiments is not specific to either free or incorporated β-tubulin IIA, so the production of free β-tubulin IIA could create increased fluorescence, even in a cell with reduced stable MTs. Our gross morphological findings of TORC1 LOF coincide ...
Due to a lack of an appropriate ELISA kit or antibody, the ECS-complex component elongin C was not analyzed in this study. 2.4. Statistics All data are given as mean ± standard deviation (SD) unless stated otherwise. Pearson correlation coefficients and unpaired t-tests were calculated using...