aWestern blot analysis of gametocyte lysates from lines expressing endogenously HA-tagged alleles of core SCF components: RBX1-HA and SKP1-HA.bLocalisation of RBX1-HA and SKP1-HA (green) by widefield immunofluorescence in micro- and macro-gametocytes between two- and 12-min post-activation. ...
Clear cell renal cell carcinoma (ccRCC) is intimately associated with defects in ubiquitin-mediated protein degradation. Herein, we report that deficiency in the E3 ligase subunit cullin 5 (CUL5) promotes chromosomal instability and is an independent neg
To identify brain regions where Rbx2 regulates Dab1 turnover, we performed immunofluorescence on rbx2 cKO and control littermates. Dab1 protein was increased in the neocortex, cerebellum, superior colliculus, and thalamus but not the striatum or medulla (Figure 2B). In the neocortex, Dab1 was...
b, Immunofluorescence of wild-type and PD-L1 knockout MDA-MB-231 cells using the anti-PD-L1 antibody. c, d, Immunochemistry using the anti-PD-L1 antibody of wild-type and PD-L1 knockout MDA-MB-231 cells from cultures on glass slides (c) or from implanted tumours (d). e, f, ...