aWestern blot analysis with an antibody against ubiquitin-K48 (α-Ub K48) of gametocyte lysates collected at different time points during the first 15 min following activation by xanthurenic acid. The Ponceau staining serves as a loading control.bTreatment pre-activation of gametocytes with ...
1:50-1:200 - 产品规格 种属反应 Human,Mouse,Rat 宿主/亚型 Rabbit / IgG Expression System HEK293 cells 分类 Recombinant Monoclonal 类型 Antibody 克隆号 ARC0102 抗原 A synthetic peptide corresponding to a sequence within amino acids 669-768 of human Cullin 3 (Q13618) ...
1:2,000 - 产品规格 种属反应 Dog, Human, Mouse, Non-human primate, Rat 宿主/亚型 Mouse / IgG1 分类 Monoclonal 类型 Antibody 克隆号 OTI7F8 抗原 Human recombinant protein fragment corresponding to amino acids 1-300 of CUL4B produced in E.coli 查看免疫原 偶联物 Unconjugated...
The presence of both Cul1 and Skp2 in the same fractions reflects the assembled CRL1 complexes which peak in CAND1 knockdown cells. The anti-CSN8 antibody marks the position of the CSN complex. Density gradients were calibrated with purified CSN and 20S proteasome. Download: Download high-res...
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Antibody Chip Apoptosis Assays 96 Wells Blog Blotting Buffer Ca+ Cell Culture Cell Death Colour Cell Extract Cellular Extract Comprehensive proteome analysis of an Apc mouse model uncovers proteins associated with intestinal tumorigenesis Cul3-mediated Nrf2 ubiquitination and antioxidant response element (ARE...
cant. anti-human GAPDH polyclonal antibody (diluted 1:1000) and mouse anti-human Cullin1 monoclonal antibody (diluted 1:500) from Santa Cruz Biotechnology, USA, and later horseradish peroxidase-linked IgG as the secondary antibodies. The band density was measured with a computer-assisted image-...
Clear cell renal cell carcinoma (ccRCC) is intimately associated with defects in ubiquitin-mediated protein degradation. Herein, we report that deficiency in the E3 ligase subunit cullin 5 (CUL5) promotes chromosomal instability and is an independent neg
CAND1 dissociation, the first is neddylation, and the second is the binding of additional CRL components. Neddylation was initially shown to dissociate CAND1 based onin vitroexperiments with endogenous human CUL1 that was bound to antibody after immunoprecipitation [112]. However, studies using ...