R., Ghorani, E., Solomon, I., Lesko, M.H., Ruef, N., Roddie, C., et al.; TRACERx Melanoma; TRACERx Renal; TRACERx Lung consortia (2018). Fc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies.Cancer Cell 33, 649–663.e4.6...
CTLA-4阻断可显著延长4T1-KD小鼠的存活时间,但不能延长4T1-SC荷瘤小鼠的存活时间。为了阐明这种效应的免疫学性质,我们在抗CTLA-4治疗和手术切除4T1-KD肿瘤后大约100天重新植入了4T1-KD或4T1-KO细胞, 4T1-KD肿瘤小鼠和4T1-Sc肿瘤小鼠的肿...
差异基因表达(DEG)的分析结果表明,与抗CTLA-4 m2a抗体治疗组相比,DT治疗组中差异表达的基因非常少,并且都与I型IFN信号通路无关。此外,如果敲除小鼠的FcγR基因(FcgrKO小鼠),抗CTLA-4 m2a抗体也不能抑制其肿瘤生长和驱动髓系细胞重编程。 也就是说,在抗CTLA-4 m2a抗体治疗后,FcγR结合在驱动髓系细胞重编程...
差异基因表达(DEG)的分析结果表明,与抗CTLA-4 m2a抗体治疗组相比,DT治疗组中差异表达的基因非常少,并且都与I型IFN信号通路无关。此外,如果敲除小鼠的FcγR基因(FcgrKO小鼠),抗CTLA-4 m2a抗体也不能抑制其肿瘤生长和驱动髓系细胞重编程。 也就是说,在抗CTLA-4 m2a抗体治疗后,FcγR结合在驱动髓系细胞重编程...
BMDM分别来自野生型(WT)、FcgrIfnar) KO小鼠。此外,他们还将Treg细胞和BMDM与抗CTLA-4 m1抗体、抗CTLA-4 m2a抗体和对照的培养基分别进行共培养,并对分选出的BMDM进行scRNA-seq。 研究发现,体外培养实验的结果与小鼠体内实验的结果一致。在野生型小鼠中,与对照和抗CTLA-4 m1抗体治疗组相比,在抗CTLA-4 m2a抗体...
为了进一步确证，从WT/CTLA4KO小鼠的嵌合骨髓中分离Treg细胞，体外和CHO-GFP-CD80细胞进行孵育，结果显示CD80的被内吞过程依赖于CTLA-4，并且只发生在效应Treg细胞上（CTLA4表达更高）。进一步用BMDC-GFP-CD80细胞和OVA-TCR-Treg细胞共孵育，结果显示在OVA存在下会发生梯度浓度的CD80“丢失”，而CTLA-4抗体可以阻断...
4. F. Arce Vargas, A. J. S. Furness, K. Litchfield, K. Joshi, R. Rosenthal, E. Ghorani, I. Solomon, M. H. Lesko, N. Ruef, C. Roddie, J. Y. Henry, L. Spain, A. Ben Aissa, A. Georgiou, Y. N. S. Wong, M. Smith, D. Strauss, A. Hayes, D. Nicol, T. O'...
Ko uki T ,Sawai Y ,Gardine CA ,et a1.c 兀A _4 gene polymo r- p 锄 at position 4 9 in exon 1 reduces the inhibitory functi on of C兀A .4 and contributes to th e pathogenes is of G raves ’disel丑se . J Immunol,20 oo,165 :6 606 ...
Furthermore, in vivo delivery of CTLA-4 WT and knockout (KO) Tregs into CD45.1+RAG2 KO mice showed that transfer of congenic CD45.1 protein from host cells to donor Tregs was significantly diminished in CTLA-4 KO Tregs compared to WT Tregs. The tail portion of CTLA-4 was also found ...
通过hPD-L1(NM-HU-00062)与hCTLA4(NM-HU-00014)交配获得。 应用领域:免疫治疗;肿瘤研究;药物筛选 *使用本品系发表的文献需注明: hPD-L1/hCTLA-4 mice (Cat. NO. NM-HU-00102) were purchased from Shanghai Model Organisms Center, Inc.. 验证数据 ...