Cota-S佗nchez J H, Remarchuk K, Ubayasena K. Ready-to- use DNA extracted with a CTAB method adapted for herbarium specimens and mucilaginous plant tissue [J]. Plant Molecular Biology Reporter, 2006, 24: 161-167.Cota-Sanchez JH, Remarchuk K, Ubayasena K (2006) Ready-to-use DNA ...
Unlike the animal cell, DNA extraction from plant cells faces challenges of a rigid cellulose cell wall and the presence of a variety of cytoplasmic components like polysaccharides, polyphenols, lipids, and proteins. This demands modification in the DNA extraction protocol to obtain DNA in the pures...
frozen quickly and thawed only in the presence of an extraction buffer that contains detergent and a high concentration of EDTA.Materials Plant tissue, freshLiquid nitrogen2× CTAB extraction buffer24:1 (v/v) chloroform: isoamyl alcoholIsopropanolWash buffer95% EtOHTE buffer, pH 8.010 mg/mL DNa...
Reviews of Isolation of Plant DNA from fresh tissue This chapter discusses the determination plant tyrosinase. The darkening of mushrooms, potatoes, apples, and many other plants and plant products on injury to the tissue is the result of the enzymatic oxidation of certain mo/aohydric and... 被...
CTAB法小量制备植物基因组DNA 【原理】 CTAB分离DNA的方法最初用于细菌，后来经修改用于从植物中获取DNA。CTAB（即十六烷基三乙基溴化铵）是一种阳离子去污剂。CTAB可与核酸形成复合物，该复合物仅溶于高盐溶液。此外，CTAB可十分有效地促进DNA和RNA与多糖的分离并去除多糖。可通过提高NaCl的浓度和沉淀核酸去除CTAB。
Therefore, proper handling of plant tissue samples and storage to maximize shelf life is crucial in molecular and diagnostic studies. The cetyltrimethylammonium bromide (CTAB) method of nucleic acid extraction has become a reliable method for many applications in plant science in recent years. Murray...
AD Sharma,PK Gill,P Singh - 《Plant Molecular Biology Reporter》 被引量: 301发表: 2002年 The relationship between genetic and cytogenetic maps of pea. II. Physical maps of linkage mapping populations DNA was extracted from leaf tissue using a CTAB method as described by Rogers and Bendich [...
throughputgenomicstudies.AscomparedwiththemodifiedCTABDNAextractionmethod,theless expensivehighsalt,lowpHmethod(HSLPM)withminormodificationsyieldsmuchmorepeanut DNA,over1000gpergramofleaftissuesonafreshweightbasis,withslightlylowermolecular weight,buttheRAPD-PCRpatternmakesnodifference,andtheDNAcanbecompletelydigested...
We have CTAB (Cetyl tri methyl ammonium bromide) method for isolation of DNA from plant tissue. The method is suitable for isolating DNA from a small to mediumnumber of plant samples. The DNA can be stored for a longer duration. The method involves extraction of DNA using a buffer (pH ...
Results from 60 plants were examined, and were consistently amplifiable in the RAPD reaction with as little as 0.5 ng DNA per 25-渭L reaction. Presently, this is the first procedure for the isolation of DNA from mature strawberry leaf tissue that produces consistent results for a variety of ...