The variability in the cell wall structure and the level of secondary metabolites in the plant cells interfere with the DNA extraction. The DNA extraction protocol standardized for one plant species may vary for other. However, the CTAB (Cetyl Trimethyl Ammonium Bromide) method of DNA extraction ...
本试剂盒采用可以特异性结合DNA的离心吸附柱和独特的缓冲液系统,用于提取植物细胞中的基因组DNA。离心吸附柱中采用的硅基质材料为本公司特有新型材料,高效、专一吸附DNA,可最大限度去除植物细胞中杂质蛋白及其他有机化合物。提取的基因组DNA片段大,纯度高,质量稳定可靠。
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(CTAB)-based protocol for the extraction of genomic DNA from a range of medicinal plant species, namely Sclerocarya birrea (tree), Barleria greenii (... M Moyo,SO Amoo,MW Bairu,... - 《South African Journal of Botany》 被引量: 60发表: 2008年 FT-Raman signatures of genomic DNA from ...
中文名称:CTAB植物基因组DNA快速提取试剂盒(离心柱型) 中文同义词:CTAB植物基因组DNA快速提取试剂盒(离心柱型) 英文名称:CTAB Plant Genomic DNA Rapid Extraction Kit (Spin Column Type) 英文同义词:CTAB Plant Genomic DNA Rapid Extraction Kit (Spin Column Type) ...
and proteins. This demands modification in the DNA extraction protocol to obtain DNA in the purest form possible. The major modification includes the replacement of SDS with CTAB, which, apart from being a detergent, helps in the precipitation of polysaccharides under high salt concentrations. Additi...
quantities and stored at -80℃ for DNA extraction. Genomic DNA was extracted by CTAB method [38]. The quality and concentration of DNA were determined by Nanodrop One ultra-micro ultraviolet spectrophotometer, and the final concentration of DNA was adjusted to 100 ng/μL for subsequent ...
clean roots were collected to extract the DNA of the endophyte microbiome using the CTAB method [57]. Bulk soil was collected from sites without plants and near the selected trees. Then, the bulk soil samples were immediately frozen on dry ice and transferred to the laboratory. In total, we...
Future genomic studies will rely on optimized CTAB protocols. Our review of the modifications that have been used, as well as the protocols we provide here, could better standardize DNA extractions, allowing for repeatable and transparent studies....
Genomic DNA extraction was performed using a rapid cetyl-trimethyl- ammonium bromide (CTAB) method [53]. We used a combination of three sequencing methods (target-enrichment sequencing, low-coverage whole genome sequencing (WGS), and DArTseq) to capture variation in and around the gene-containing...