The variability in the cell wall structure and the level of secondary metabolites in the plant cells interfere with the DNA extraction. The DNA extraction protocol standardized for one plant species may vary for other. However, the CTAB (Cetyl Trimethyl Ammonium Bromide) method of DNA extraction ...
本试剂盒采用可以特异性结合DNA的离心吸附柱和独特的缓冲液系统,用于提取植物细胞中的基因组DNA。离心吸附柱中采用的硅基质材料为本公司特有新型材料,高效、专一吸附DNA,可最大限度去除植物细胞中杂质蛋白及其他有机化合物。提取的基因组DNA片段大,纯度高,质量稳定可靠。
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[Objective]The research aimed to optimize the extraction methods for genomic DNA in Eomecon chinantha Hance.. [Method]The methods of CTAB and modified CTAB... HU Xue-Hua,X Chen,XJ Liao,... - 《Medicinal Plant》 被引量: 15发表: 2010年 Comparative Analysis on Methods for Genomic DNA Ex...
中文名称: M5 HIPER CTAB植物基因组DNA快速提取试剂盒 中文同义词: M5 HIPER CTAB植物基因组DNA快速提取试剂盒 英文名称: M5 HIPER CTAB Plant Genomic DNA Rapid Extraction Kit 英文同义词: M5 HIPER CTAB Plant Genomic DNA Rapid Extraction Kit CAS号: 分子式: 分子量: 0 EINECS号: 信息错误报告 您的...
cetyltrimethylammonium bromide (CTAB)extractiongenomic DNAnextゞeneration sequencingplantPremise Commonly used molecular techniques such as nextゞeneration sequencing require reliable methods to extract DNA quickly and efficiently. Secondary compounds within plant tissues make this requirement all the more ...
We generated 27 iSTU-CRISPR/Cas9 T-DNA vectors with all possible combination of three intron systems (inS, inO and inR) and three sgRNA processing systems (NU, tRNA and RZ) at three target sites. We transfected these constructs into rice protoplasts. The results based on restriction fragment ...
The CRISPR-Cas system, an innate immune system in many bacteria and archaea, has been harnessed to develop genome engineering tools. Since the first demonstration of CRISPR-Cas9 for inducing targeted DNA double-strand breaks (DSBs) in prokaryote and eukaryote cells1,2, this powerful tool has rev...
Total genomic DNA was extracted using a cetyltrimethylammonium bromide (CTAB) method24. DNA quantity and quality were determined using a Qubit 4.0 fluorometer. Short read sequencing libraries with ~350 bp insert size were constructed and sequenced on the Illumina NovaSeq 6000 platform to generate ...
DNA extraction, GBS and SNP calling Genomic DNA was isolated from the leaf of two-week-old moth bean seedlings using CTAB (cetyl trimethyl ammonium bromide) method [59] and treated with RNase to remove RNA contamination. The integrity of DNA samples was tested on 0.8% agarose gel and concent...