High contrast cryoEM grid storage boxes for holding clipped or unclipped grids. Designed to fit grid box sockets of the FEI Vitrobot™, the Gatan Cryo Workingstation™, FEI clipping and loading station, Leica plunger and most other current cryo-TEM devices and stations. This product is not...
CryoEM grids of PN45545 TCR-CD3 without antigen were prepared at a protein concentration of ~2 mg/mL. Complex of PN45545 TCR-CD3 and MAGEA4 pMHC/2M2 Fab was obtained by mixing the two components at concentrations of ~0.6 mg/mL and ~0.75 mg/mL, respectively, and incubating on...
MS-rings that could not be classified correlated with grids that had thinner ice, suggesting that the MS-ring preferentially partitions at the air–water interface. However, we cannot exclude the presence of other stoichiometries that we could not classify. Fig. 6: Torque transmission during ...
2.5 Current strategies for optimizing preparation of cryo-grids. Air-water interface and physics of thin films. How to troubleshoot. Review of different freezing hardware devices. 3 Data collection 3.2 Radiation damage in cryo-EM. Sources of radiation damage, role of electron energy, exposure weigh...
Cryoet of Single Particle CryoEM Grids Reveals Widespread, but Reducible, Particle Adsorption to the Air-Water Interfacedoi:10.1016/j.bpj.2018.11.099Alex J. NobleVenkata P. DandeyHui WeiJulia BraschJillian ChasePriyamvada AcharyaYong Zi Tan...
A recent systematic study of particle localization on cryoEM grids prepared with traditional blotting methods by Noble et al. (2018a) has shown that ∼90% of the 46 samples analyzed associate with the AWI, demonstrating that the vast majority of specimens have the potential to be perturbed by...
All the three grids showed very similar diffraction peak intensities from both of the lattice planes, and the intensity ratios before and after oxidation/ epoxidation were ~ 1.0 (Supplementary Fig. 2f.), indicating that little damage was caused to graphene on the EG-grid during C ...
This article explores the use of lipid-monolayers affinity grids for cryo-electron microscopy (CryoEM) structural determination of protein complexes. The study investigates the feasibility of using Ni-NTA lipid monolayers to bind proteins with a poly-histidine purification tag. The researchers provide ...
By using a rapid plunge-freezing robot and nanowire grids, we were able to reduce some of the deleterious effects of the air-water interface by decreasing the dwell time of particles in thin liquid films. We demonstrated this by using single-particle cryo-EM and cryo-electron tomography (cryo...
GRIDS (Cartography)THIN filmsDATA qualityACQUISITION of dataSURFACE active agentsSample preparation can present a significant hurdle within single particle cryo-electron microscopy (cryoEM), resulting in issues with reproducibility, data quality or an inability to visualise the sample. There...