Single particle cryo em is a powerful structural biology technique for 3D characterization of proteins, protein complexes and macromolecules at near-atomic and atomic resolution.
Single particle cryo em is a powerful structural biology technique for 3D characterization of proteins, protein complexes and macromolecules at near-atomic and atomic resolution.
Single particle cryo em is a powerful structural biology technique for 3D characterization of proteins, protein complexes and macromolecules at near-atomic and atomic resolution.
Advances in hardware and software have now opened up the technique to more widespread adoption in basic research and drug discovery. Cryo-EM is used to visualize a range of biological specimens from large cellular organelles (using cryo tomography) all the way down to the near-atomic resolution ...
Single-particle cryo-electron microscopy (cryo-EM), is an increasingly popular technique used by structural biologists to solve structures at atomic resolution. This technique complements x-ray crystallography because it reveals structural details without the need for a crystalline specimen. Through the ...
Single particle cryo-Electron Microscopy (cryo-EM) is a technique that allows the structural characterization of macromolecules without the need for crystallization. For certain type of samples that are ideally suited for cryo-EM studies it has been possible to reach high-resolution structures ...
Cryo-electron microscopy (Cryo-EM) is a technique that uses accelerated electrons as illumination source for biological samples. To minimize radiation damage, the sample is kept at cryogenic temperatures and the electron dose has to be low – resulting in noisy images. However, the enormous progres...
Single particle electron cryo-microscopy (cryo-EM) is a powerful technique to determine the structures of protein complexes down to atomic resolution. The sample in solution is flash-frozen in liquid ethane, forming a thin layer of vitreous ice, in which the sample particles are ideally evenly ...
The limitations with X-ray crystallography and NMR to solve SHR protein structures prompted investigators to move towards cryo-EM technique. The cryo-EM structural analyses have been successful in revealing structural dynamics of several SHRs in recent years. Though, limited by low-resolution, the ...
Cryo-electron microscopy (cryo-EM) is a powerful technique for determining the structures of biological macromolecular complexes. Picking single-protein particles from cryo-EM micrographs is a crucial step in reconstructing protein structures. However, the widely used template-based particle picking process...