Single particle cryo em is a powerful structural biology technique for 3D characterization of proteins, protein complexes and macromolecules at near-atomic and atomic resolution.
Single particle cryo em is a powerful structural biology technique for 3D characterization of proteins, protein complexes and macromolecules at near-atomic and atomic resolution.
Single particle cryo em is a powerful structural biology technique for 3D characterization of proteins, protein complexes and macromolecules at near-atomic and atomic resolution.
Since MicroED is a diffraction technique, samples need to be crystalline, and the crystallization process is the same as for X-ray crystallography. However, much smaller crystals can be used in MicroED because the interaction of the crystal with electrons is much stronger than it is with X-rays...
Advances in hardware and software have now opened up the technique to more widespread adoption in basic research and drug discovery. Cryo-EM is used to visualize a range of biological specimens from large cellular organelles (using cryo tomography) all the way down to the near-atomic resolution ...
Cryo-electron microscopy (Cryo-EM) is a technique that uses accelerated electrons as illumination source for biological samples. To minimize radiation damage, the sample is kept at cryogenic temperatures and the electron dose has to be low – resulting in noisy images. However, the enormous ...
Abstract Microcrystal electron diffraction (MicroED) has advanced structural methods across a range of sample types, from small molecules to proteins. This cryogenic electron microscopy (cryo-EM) technique involves the continuous rotation of small 3D crystals in the electron beam, while a high-speed ...
Single particle electron cryo-microscopy (cryo-EM) is a powerful technique to determine the structures of protein complexes down to atomic resolution. The sample in solution is flash-frozen in liquid ethane, forming a thin layer of vitreous ice, in which the sample particles are ideally evenly ...
Cryo-EM Vitrification Starter KitSingle particle electron cryo-microscopy (cryo-EM) is a powerful technique to determine the structures of protein complexes down to atomic resolution. The sample in solution is flash-frozen in liquid ethane, forming a thin layer of vitreous ice, in which the ...
Single-particle cryo-electron microscopy (cryo-EM), is an increasingly popular technique used by structural biologists to solve structures at atomic resolution. This technique complements x-ray crystallography because it reveals structural details without the need for a crystalline specimen. Through the ...