Here, we present a structural snapshot of SARS-CoV-2 RTC as it transitions toward cap structure synthesis. We determine the atomic cryo-EM structure of an extended RTC assembled by nsp7-nsp82-nsp12-nsp132-RNA and a single RNA-binding protein, nsp9. Nsp9 binds tightly to nsp12 (RdRp)...
Cryo-EM structure of an extended SARS-CoV-2 replication and transcription complex reveals an intermediate state in cap synthesis. Cell 184, 184–193.e10 (2021). Article CAS PubMed Google Scholar Hillen, H. S. Structure and function of SARS-CoV-2 polymerase. Curr. Opin. Virol. 48, 82–...
Here we used cryo-electron microscopy (cryo-EM) to determine the structure of the post-fusion SARS-CoV S2 trimer. We present an analysis of the N-linked glycan shield masking the surface of the S2 machinery. Comparison with the structure of the pre-fusion SARS-CoV S glycoprotein reveals dra...
SARS-CoV-2COVID-19cryoEMcryoETinactivated virusvaccinespikesubtomogram averagingprefusionpostfusionThe outbreak of SARS-CoV-2 in December 2019, led to the ongoing global pandemic of coronavirus disease 2019 (COVID19), which has claimed more than a half milli...
Since the outbreak of the COVID-19 pandemic, cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET) have been demonstrated to be powerful and efficient tools in structural studies of distinct conformational states of the trimeric spike protein of SARS-CoV-2 and the VOCs as ...
The structure provides structural definition of the functionally critical membrane-interacting segments, including the fusion peptide and transmembrane anchor. The internal fusion peptide forms a hairpin-like wedge that spans almost the entire lipid bilayer and the transmembrane segment wraps around the ...
SARS-CoV-2QUATERNARY structureSARS-CoV-2 Omicron variantGLYCANSMONOCLONAL antibodiesPEPTIDESThe monomeric human ACE2-PD exhibited binding to the RBDs of BA.2, BA.5, BA.2.75, BF.7, and XBB.1 with binding affinity ( I K i SB D sb ) of 4.80 ± 0.01, 3.2 ±...
The BA.2 sub-lineage of the SARS-CoV-2 Omicron variant has gained in proportion relative to BA.1. As differences in spike (S) proteins may underlie differencesStalls, VictoriaLindenberger, JaredGobeil, SophieHenderson, RoryParks, RobBarr, Maggie...
Deletion of 3a in SARS-CoV-1 reduces viral titer and morbidity in mice, suggesting it could be an effective target for vaccines or therapeutics. Here, we present structures of SARS-CoV-2 3a determined by cryo-EM to 2.1- resolution. 3a adopts a new fold with a polar cavity that opens ...
Structure of an RNA G-quadruplex from the West Nile virus genome J. Ross Terrell Thao T. Le Jessica L. Siemer Nature Communications(2024) SARS-CoV-2 biology and host interactions Silvio Steiner Annika Kratzel Volker Thiel Nature Reviews Microbiology(2024) ...