https://www.youtube.com/watch?v=Asp2Sg-AN-gWe show how to start building an atomic model into a cryoEM map using an atomic structure from the AlphaFold database. We fit the atomic model into the map a, 视频播放量 62、弹幕量 0、点赞数 5、投硬币枚数 1、收
但是由于结合抗体的多克隆性质和cryoEMPEM map的分辨率约为3到4埃,仅凭结构信息难以直接确定抗体序列。==作者假设如果能够获取适当的序列数据库==,在这个序列数据库中包含与采集血清(即pAb)的时间点相匹配的B细胞受体库(BCR),那么就可以利用cryoEMPEM中的含有的pAb的结构信息,从BCR库中选择出重链和轻链的候选者。
density-modification procedure was applied to a set of 104 datasets and improved map-model correlation and increased the visibility of details in many of the maps. The procedure requires two unmasked half-maps and a sequence file or other source of information on the volume of the macromolecule ...
Predicted models of LayV-G was first obtained using Alphafold266, which was further manually adjusted based on the cryo-EM map with Coot 0.8.267. Each residue was manually checked with the chemical properties taken into consideration during model building. Several segments, whose corresponding dens...
2024年2月23日,来自华中科技大学黄胜友等研究人员合作在学术期刊《自然—生物技术》上发表了标题为“All-atom RNA structure determination from cryo-EM maps.”的研究成果,实现根据冷冻电镜图谱确定全原子RNA结构。 研究人员表示,从冷冻电镜图谱中确定蛋白质结构的方法很多,但对于RNA结构来说,这仍然是一项挑战。
Post-process input map path/to/inputVol.mrc and save it at path/to/outputVol.mrc using high resolution deep learning model located in path/to/deep/learningModel deepemhancer -p highRes --deepLearningModelDir path/to/deep/learningModel -i path/to/inputVol.mrc -o path/to/outputVol.mrc ...
The first is the local FSC (Fourier shell correlation) between the full map and the model, while the second is calculated between the half maps normally used in typical single particle analysis workflows. We call the quality measure "FSC-Q", and it is a quantitative estimation of how much ...
Since the high density in a map typically follows the mainヽhain of the protein, the mainヽhain connection between secondary structure elements can often be identified as the unbranched path between them with the highest minimum value along the path. This chain‐tracing procedure is then combined...
cryodrgn backproject_voxel now produces half-maps and a half-map FSC fixing filter_star to accept tilt series as well fixing assorted bugs in e.g. write_star, invert_constrast, and train_vae (see release notes) Version 3.1 cryodrgn filter interface for interactive filtering of particles as ...
cryo-EM, scanning transmission electron microscopy (STEM), or for resolving elements of large structures by TEM in conjunction with image processing. Undecagold has been used to see the biotin binding sites on avidin to 1 nm resolution by electron microscopy. It is prepared in a form with one...