近日,来自字节跳动 ByteDance Research 团队的研究人员提出了 CryoSTAR 方法,成功将来自原子模型的结构先验应用到冷冻电镜实验数据的动态解析中,为解决这一问题带来了新的思路和方法。该成果相关论文 “CryoSTAR: Leveraging Structural Prior and Constraints for Cryo-EM Heterogeneous Reconstruction” 已刊发于 Nature 旗...
Recent advances in single-particle cryo-electron microscopy (cryo-EM) have enabled structure determinations of RNA to atomic resolutions. However, most RNA molecules are structurally flexible, limiting the resolution of their structures solved by cryo-EM. In modeling these molecules, several ...
This strategy significantly improves the efficiency of RMSF-net when dealing with large cryo-EM maps. The retained box indices are recorded for the subsequent merging algorithm. In addition, the voxel densities within the boxes are normalized to a range of [0, 1] before being input into the ...
Caution was taken not to mask out the densities for the bound ligand and lipids. The map was then extended by 2 pixels and supplied with a soft edge width of 12 pixels using relion_mask_create. g, Local-resolution map for the 3D EM reconstruction of Cav3.1-Δ8b in the presence of Z...
If no such structures exist, and the EM reconstruction does not reach a resolution of 9 Å, where α helices become visible as rod-shaped densities, it may be difficult to distinguish correct reconstructions from incorrect ones. For this purpose, a robust validation method was introduced that ...
extra densities were observed near the contact sites of two protofilaments (Supplementary Fig.3b). At the current resolution of the 3D maps, we cannot comment on the origin of these extra densities. However, it can be possible that they arise due to terminal regions of the fibril protein. ...
We did not resolve densities in our structures that can be interpreted as bound PIP2. However, our earlier studies showed that the N-terminal segment bearing a cluster of positively charged residues is crucial for PIP2 modulation of TMEM16F channel activity (Ye et al., 2018). In our structu...
These cryo-EM structures represented a homodimer of ClC-2 proteins. The overall architectures of these two structures were generally the same, except for the additional cryo-EM density of the AK-42 inhibitor in the complex (Supplementary Figs. 3, 4d). The two maps displayed clear densities of...
Resolving conformational heterogeneity in cryogenic electron microscopy datasets remains an important challenge in structural biology. Previous methods have often been restricted to working exclusively on volumetric densities, neglecting the potential of incorporating any preexisting structural knowledge as prior ...
0.2 mg/ml of protein concentration).Error bars are the standard deviation of crystal domain sizes with n¼20. (F)The dependence of the average liposome density on the incubation time.The lines represent the liposome densities on 2D crystal (solid line)and on a continuous carbon support film...