深圳易致生物科技有限公司,基于DNA/RNA快速扩增技术,设计、研发与转化病原微生物快速检测产品。拳头产品:超敏胰岛素ELISA试剂盒,支原体检测试纸条,支原体清除剂,支原体预防剂,恒温PCR,CRISPR,Cas12a, Cas13a
crRNA Design-Cas12a(Cpf1) Input Sequence General Cpf1 Setting Primers Options Input an DNA sequence in FASTA format OR upload a fasta file: Choose File no file selected Find targets
这将打开显示该特定gRNA的潜在脱靶位点的页面,甚至提供可用于通过SURVEYOR测定来筛选这些脱靶位点潜在突变的引物序列。 CRISPR Design 使用CRISPR Design的主要优势在于能够提供所有潜在gRNA的脱靶目标的详细信息。 它使每一个gRNA序列都发生了突变,并提供了关于其脱靶位置和与设计的gRNA错配数目的详细报告。 当设计两个gRN...
Trans-Activating crRNA is a type of guide RNA that forms a binary complex with Cas9 to target specific sequences in double-stranded DNA for genome editing. AI generated definition based on: Methods in Enzymology, 2015 Featured on this page Featured on this page Chapters and Articles You might...
CRRNA:TRACRRNA-BASED BINARY LOGIC GATE DESIGN AS A TOOL FOR SYNTHETIC BIOLOGYThe invention relates to logic-gate-based Type II or Type V CRISPR-Cas constructs and methods for modifying gene expression using the CRISPR-Cas constructs and CRISPR-Cas effector proteins.STEPANOVA, ANNA...
By combining design constraints around the programmability of the spacer sequence and its downstream repeat region, we can infer that the sequence of the whole crRNA can be altered without destroying the function of the CRISPR/Cas9 system. Subsequently, we deduced that any RNA may become a crRNA...
Based on the crystal structure of LbCas12a-crRNA-dsDNA (PDB ID: 5XUS)12, we reasoned that crRNA extensions can influence the trans-cleavage activity by either activating or inhibiting the catalytic efficiency of Cas12a, allowing us to better understand crRNA design with tunable trans-cleavage act...
For crRNAs targeting PTEN and KRAS, they were selected using the Broad Institute online sgRNA design tool (https://portals.broadinstitute.org/gpp/public/analysis-tools/sgrna-design) [17]. RNA transfection and cell viability assay Each RNA oligos suspended in RNAase-free water at a stock ...
Guo X. et al. (2021) Transcriptome-wide Cas13 guide RNA design for model organisms and viral RNA pathogens. Cell Genomics. 1;100001. 核酸研究,找艾博思! 现已开发流式细胞仪可识别的最多由40余种荧光编码微球组成的阵列,并在此检测上进一步研发灵活便捷的多细...
These newly identified features will facilitate the design and optimization of CRISPR-Cpf1-based genome-editing strategies.doi:10.1016/j.ymthe.2018.08.021Lin, LiHe, XiubinZhao, TianyuanGu, LingkaiLiu, YeqingLiu, XiaoyuLiu, HongyanYang, Fayu...