深圳易致生物科技有限公司,基于DNA/RNA快速扩增技术,设计、研发与转化病原微生物快速检测产品。拳头产品:超敏胰岛素ELISA试剂盒,支原体检测试纸条,支原体清除剂,支原体预防剂,恒温PCR,CRISPR,Cas12a, Cas13a
crRNA Design-Cas12a(Cpf1) Input Sequence General Cpf1 Setting Primers Options Input an DNA sequence in FASTA format OR upload a fasta file: Choose File no file selected Find targets
Here, we report the design and assessment of an array of 42 types of engineeredsp. Cpf1 (AsCpf1) CRISPR RNA (crRNA) and 5 types of AsCpf1 mRNA in human cell lines. We show that the top-performing modified crRNA (cr3′5F, containing five 2′-fluoro ribose at the 3′ terminus) and...
This feature could simplify the design and delivery of genome-editing tools. For example, the shorter (∼42 nt) crRNA employed by Cpf1 has practical advantages over the long (∼100 nt) guide RNA in Cas9-based systems because shorter RNA oligos are significantly easier and cheaper to ...
Cpf1, a type-V CRISPR–Cas effector endonuclease, exhibits gene-editing activity in human cells through a single RNA-guided approach. Here, we report the design and assessment of an array of 42 types of engineeredAcidaminococcussp. Cpf1 (AsCpf1) CRISPR RNA (crRNA) and 5 types of AsCpf1 ...
由于cpf1的rnase活性能够从单一转录物加工多个crrna,因此基于cpf1的转录调控系统相对于众所周知的基于cas9的系统具有优势,即,它可以轻松应用于多重基因调控。 然而,基于cpf1的转录激活系统目前仅适用于哺乳动物细胞系统(tak等(2017),使用基于crispr/cpf1的转录因子进行诱导和多重基因调控(inducibleandmultiplexgene...
野生型AsCpf1与以下比较:(A)T167/T539变体,使用靶向EGFP中的位点的crRNA;(B)S170和E174变体,使用靶向EGFP中的位点的crRNA;(C和D)S542变体,使用靶向EGFP中的位点(子图C)或内源人类基因位点(子图D)的crRNA;(E)N551和N552变体,使用靶向EGFP中的位点的crRNA;(F)K607变体,使用靶向EGFP中的位点的crRNA;(G和...
crRNA and sgRNA target site design Target sites were designed using an updated version of CRISPRscan (crisprscan.org) tool20. sgRNAs (5′GGN18-19GG) and crRNAs (5′TTTVN23) target sites without predicted off-targets were used2, 22. RNA generation crRNA and pre-crRNA DNA template were gen...
Guide RNA design The analysis of the structures and biogenesis pathways of crRNAs is imperative for the practical application of genome editing approaches. Programming Cpf1 for genome editing implementation needs the expression or delivery of crRNA alone. The spacer-derived segment of the gRNAs is si...
Experimental design and work flow. a Genome editing efficiency at selected 12 Cas9 and three Cpf1 target sites in T0 rice plants. The x-axis shows the names of sgRNAs and crRNAs which are denoted as Cas9-A to Cas9-K and Cpf1-A to Cpf1-C. The numbers of T0 and/or T1 lines that...