CRISPR-Cpf1系统已成功应用于哺乳动物,植物,细菌等多种生物体的基因组编辑。此外CRISPR-Cpf1非常适合多重基因编辑。 然而,CRISPR-Cpf1系统的靶标效率在不同的gRNA序列之间差异较大。 2019年01月07发表在Bioinformatics上的“CRISPR-DT: designing gRNAs for the CRISPR-Cpf1 system with improved target efficiency ...
We have developed the first CRISPR-Cpf1 web service application, CRISPR-DT (CRISPR DNA Targeting), to help users design optimal gRNAs for the CRISPR-Cpf1 system by considering both target efficiency and specificity. CRISPR-DT will empower researchers in genome editing. CRISPR-DT, mainly ...
We reanalyzed the published CRISPR-Cpf1 gRNAs data and found many sequence and structural features related to their target efficiency. Using machine learning technology, a SVM model was created to predict target efficiency for any given gRNAs. We have developed the first web service application, ...