Re-engineering CRISPR-Cas9 tools Utilizing CRISPR-Cas9 beyond genome editing Evolution of second-generation CRISPR gene-editing tools CRISPR-mediated gene expression regulation CRISPR-mediated epigenome editing CRISPR-mediated live cell chromatin imaging CRISPR-mediated manipulation of chromatin topology Large-...
2022年8月3日,韩国庆尚大学植物分子生物学和生物技术研究中心Jae-Yean Kim课题组在Plant Communications在线发表了题为Engineering drought and salinity tolerance traits in crops through CRISPR-mediated genome editing: Targets, tools, ch...
CRISPR tools to control gene expression in bacteria. Microbiol. Mol. Biol. Rev. 84, e00077–19 (2020). Kim, Y. B. et al. Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat. Biotechnol. 35, 371–376 (2017). Article ...
The evolution of CRISPR tools that have been harnessed in the investigation of cancer biology. Since the inception of CRISPR-associated 9 (Cas9) gene editing in mammalian cells, there has been a rapid expansion in the field of CRISPR technology. This expansion has led to the development of var...
The rapid rise in CRISPR/Cas genome editing and associated applications has led to the development of numerous computational tools for effective sgRNA design. In this chapter, we discuss the essentials of gRNA design and provide an overview of the design process. In addition to summarizing factors...
The Dharmacon reagents portfolio encompass the broadest range of quality molecular biology tools for reliable and accurate gene function manipulation supporting gene-modulation (RNAi, (siRNA / shRNA / miRNA), CRISPR modulation, (CRISPRa / CRISPRi) and ge
[3]. 2Cai Ruijie,Lv Runyu,Shi Xin'e et al. CRISPR/dCas9 Tools: Epigenetic Mechanism and Application in Gene Transcriptional Regulation.[J] .Int J Mol Sci, 2023, 24: undefined. [4]. DNMAT3 Vojta Aleksandar,Dobrinić Paula,Tadić Vanja et al. Repurposing the CRISPR-Cas9 system for ...
Save your guides as oligos on Benchling, organize them using tags and folders, and clone them into CRISPR plasmids Alternatively, export your guides, along with scores and off-target sites, for easy use in other tools Explore more resources...
we used this variant to adapt existing Cas13 mammalian cell culture applications forDrosophilacells, such as transcript tracking and RNA modification [23,47,48,49,50,51,52]. These ex vivo procedures formed the basis for generating a collection of transgenic CRISPR/Cas13 tools designed for in vi...
C. S., Small, I. D., 2011. Selection patterns on restorer-like genes reveal a conflict between nuclear and mitochondrial genomes throughout angiosperm evolution. Proc Natl Acad Sci U S A 108, 1723-8, doi:10.1073/pnas.1007667108.Hofmann, N. R., 2019. Designer PPR Proteins as Tools to...