GenCRISPR gRNA design tool is a free online tool to design individual gRNA and gRNA library. It employs a newly-developed algorithm which integrates the design instructions from the latest progress and expertise. With GenCRISPR gRNA design tool, you can design gRNAs target either 1 gene or multi...
The Alt-R CRISPR HDR Design Tool provides exceptional flexibility for seamless homology directed repair (HDR) design. Multiple input formats (gene name, accession number, genomic coordinates, sequence in FASTA format) Multiple species (human, mouse, rat, zebra fish, andC. elegans) ...
Benchling's CRISPR tool eliminates tedious work by batching gRNA design and executing it up to 100x faster than leading competitor Save and export guide sequences Save your guides as oligos on Benchling, organize them using tags and folders, and clone them into CRISPR plasmids Alternatively, export...
[11] BiocGenerics_0.42.0 crisprDesign_1.1.0 ## [13] crisprBase_1.3.0 ## ## loaded via a namespace (and not attached): ## [1] bitops_1.0-7 matrixStats_0.62.0 ## [3] bit64_4.0.5 filelock_1.0.2 ## [5] progress_1.2.2 httr_1.4.4 ## [7] tools_4.2.2 utf8_1.2.2 ## ...
(https://portals.broadinstitute.org/gpp/public/analysis-tools/sgrna-design) 较早出现的sgRNA在线设计工具,主要基于Doench等人2篇非常全面深入的sgRNA设计优化研究[4,5],以提供最高效率和最小脱靶可能的sgRNA设计。 该工具可应用于CRISPRko,CRISPRa(CRISPR activation)和CRISPRi(CRISPR interference)的sgRNA设计。提...
The improved efficiency in the design of ZFNs tremendously enhanced the capabilities to edit genomes of living cells at specifically targeted sites and opened doors for therapeutic applications of such genome-editing tools19,20. Since each zinc finger recognized a 3-bp DNA code, combinatorial ...
2.常用设计软件和设计流程 1）Broad Institute GPP 网址：https://portals.broadinstitute.org/gpp/public/analysis-tools/sgrna-design 2）Benchling 网址：https://www.benchling.com/crispr Benchling和张锋实验室设计网站都是比较全面和权威的sgRNA设计网站，以人源TP53基因为例介绍Benchling设计流程。1.首先登录https...
论文题目:DeepCRISPR: optimized CRISPR guide RNA design by deep learning 发表单位:同济大学,上海第十人民医院,阿斯利康等 论文地址:https://link.springer.com/article/10.1186/s13059-018-1459-4 代码链接:https://github.com/bm2-lab/DeepCRISPR
Brochure:CRISPR Genome Editing Tools Flyer:Invitrogen™ GeneArt™ CRISPR Nuclease Vector Kits Enhanced CRISPR/Cas9-mediated precise genome editing by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA NEWReference:Rapid and Highly Efficient Mammalian...
2. CRISPR-Cas9 experimental design 3. CRISPR-Cas9 sgRNA design 4. Genome editing outcome assessment: Sanger or next-generation sequencing 5. Use of bioinformatics tools for sgRNA design of the Tyr gene: an example 6. Applications of CRISPR-Cas9 in aquaculture 7. Discussion 8. Conclusions Declara...