CRISPR gRNA 设计网页汇总 (百度一下以下关键词,第一个结果就是) #1 CRISPR Guide RNA Design Software for Molecular Biology | Benchling #2 sgRNA Designer: CRISPRko (broadinstitute.org) #3 CRISPR RGEN Tools (rgenome.net) #4 CHOPCHOP (uib.no) #5 CRISPOR (tefor.net) #6 E-CRISP Design (e-...
会出来很多可以用的sgRNA,根据自己的需要进行选择,一般找评分靠前的位置。 备注:还有很多其它的网站,都可以使用,如:CRISPR RGEN Tools (rgenome.net);CRISPOR (tefor.net)。网站时不时会崩一下,这几个网站可以替代使用。谨慎的同学也可以联合几个网站,挑选出最合适的sgRNA。每个在线网站都有评分的侧重点,感兴趣的...
CRISPR RGEN Tools > 100 species > 20 Cas9 orthologues and Cas variants Providing multiple predictive models; downloadable and standalone; and predicting potential off-target number via Cas-OFFinder, and out-of-frame scores via Microhomology-Predictor Web server https://www.rgenome.net/cas-designer...
使用在线工具CRISPR RGEN Tools(网址:http://www.rgenome.net/cas-database/)预测靶点的特异性,如图1所示,AhMULE的sg RNA序列位于序列全长的306-328bp处。 sg RNA上下游引物设计: 以花生基因AhMULE靶点:GTTCAGGAAGGGGTATCTCACGG TGG(PAM序列)为例设计引物,引物序列如下: ...
Sequencing read fatstq files were analyzed using Cas-Analyzer (http://www.rgenome.net/cas-analyzer/), and the indel ratio (mutant DNA read number/total DNA read number) was calculated from the read number. Data and code availability CRISPR RGEN Tools is an open-source collaborative initiative...
CRISPR Rgen tools Cas-OFFinder software ( http://www.rgenome.net/cas-offinder/) was used to identify potential off-target sites for SaCas9, using a mismatch number of ≤3, DNA bulge size ≤1 and RNA bulge size ≤1. For Strategy B, genomic deep sequencing was performed on a ∼230...
The base editing efficiencies were analysed by the CRISPR RGEN tools. Extended Data Fig. 7 Undetectable off-target genome-editing activity by Cas9-Act3.0 and CBE-Cas9n-Act3.0 at the AtFT promoter with 15-nt protospacers. a, Identification of Cas9-Act3.0-mediated potential indel mutations at ...
Eventually, we used the Cas-Designer [27] and Cas-OFFinder [28] inside the CRISPR RGEN Tools (http://www.rgenome.net) which have both the whole genome sequences of the Asian tiger mosquito, Aedes albopictus, and the C6/36 cell line added on our request. The Cas-Designer returned the...
Raw reads of paired-end Miseq sequencing were joined by the programme ‘fastq-join’, and indel frequency and patterns in joined reads were analysed using the Cas-Analyzer programme implemented in CRISPR RGEN Tools (http://rgenome.ibs.re.kr). Confocal laser scanning microscopy The Cy3-...
RGEN Cas-Designer: http://www.rgenome.net/cas-designer/ Vienna Bioactivity CRISPR (VBC) score: https://www.vbc-score.org Glossary Homology-directed repair (HDR). An error-free DNA damage repair mechanism that uses an existing DNA template.Non...