今天给大家推荐一款华南农业大学刘耀光老师团队(Project Manager: Yao-Guang Liu, Xianrong Xie)开发的网页工具(阅读原文可达)——CRISPR-GE:http://skl.scau.edu.cn 。 其他行业了解不多,在咱做水稻这块,无论正向还是反向都是离不开CRISPR/Cas9载体构建的。在做分子这块,大家都是构建载体的一把好手,但总会遇到...
CRISPR-GE: A Convenient Software Toolkitfor CRISPR-Based Genome EditingDear Editor,Use of the clustered regularly interspaced short palindromicrepeat (CRISPR)-associated protein9 (Cas9) and Cpf1 systemsin plants (Ma et al., 2016; Wang et al., 2017) involves manysteps, including the selection ...
Therefore, we have developed CrisprGE, a central hub of CRISPR/Cas-based genome editing. Presently, this database holds a total of 4680 entries of 223 unique genes from 32 model and other organisms. It encompasses information about the organism, gene, target gene sequences, genetic modification...
CrisprGE: a central hub of CRISPR/Cas-based genome editing CRISPR system is a powerful defense mechanism in bacteria and archaea to provide immunity against viruses. Recently, this process found a new application i... K Karambir,T Himani,GA Kumar,... - 《Database the Journal of Biological...
Xie X, Ma X, Zhu Q, Zeng D, Li G, Liu YG (2017) CRISPR-GE: a convenient software toolkit for CRISPR-based genome editing. Mol Plant 10(9):1246-1249Xie X, Ma X, Zhu Q, Zeng D, Li G, Liu YG: CRISPR-GE: A Convenient Software Toolkit for CRISPR-Based Genome Editing. ...
Figure 1. The CRISPR-GE Toolkit for Genome Editing. (A) The overall workflow of CRISPR-GE. (B) The submission page of targetDesign. (C) The results page of targetDesign and a table of results. Low-efficiency sites with contiguous “T” bases (four or more), very low or very high ...
‹Genome Editing Support Center CRISPR-Based Genome Editing Support CRISPR-Based Genome Editing Support—Getting Started CRISPR-Based Genome Editing Support—Troubleshooting TALEN-Based Genome Editing Support Gene Synthesis and Mutagenesis Support Explore...
4日，博德研究所授予GE医疗和Sigma生物CRISPR/ Cas9相关知识产权用于研究应用。据此，GE医疗和Sigma生物将获得博德研究所的CRISPR/Cas9专利组合，其中包括由博德研究所的FengZhang开 创的利用病人真核细胞进行基因编辑的技术。此外，博德许可GE医疗发展其最新开创的DharmaconEdit-RCRISPR/Cas9基因编辑系统用于建立永久的 可...
摘要 本研究利用CRISPR/Cas9基因编辑技术对本实验室分离得到的PRV GD株的gI和gE基因进行基因敲除,经蚀斑纯化结合PCR鉴定的方法获得gI和gE基因缺失病毒,命名为PRV GD-delgI/gE。试验测定了该基因缺失病毒在PK-15细胞中培养的一步生长曲线及其对小鼠的致病力。试验结果表明,与亲本毒株PRV GD株相比,PRV GD-delgI/gE...
中国科学院微生物研究所病原室温廷益研究组与工业室何秀萍研究组合作在汉逊酵母中建立了一套CRISPR-Cas9介导的多基因编辑技术(CMGE),尤其用于多基因一步敲除、多位点(ML)、多拷贝(MC)目标基因的同时插入。由于O.polymorpha中没有稳定的游离载体,Cas9和gRNA被整合到基因组上进行表达,先将Cas9基因通过同源重组的方式整合...