该研究首先利用体外生化实验证明了CPSF30-L的YTH结构域可以优先结合带有m6A修饰的RNA,同时解析了CPSF30-L的YTH结构域结合m6A RNA的复合物结构,获得了参与m6A修饰识别的关键氨基酸位点信息(如:W259,W310和Y319);对带有m6A修饰的基因与poly(A)位点的选择受到CPSF30调控的基因关联分析,发现存在m6A修饰的基因在CPSF30突...
CPSF30-L-mediated recognition of mRNA m6A modification controls alternative polyadenylation of nitrate signaling-related gene transcripts in Arabidopsis m^(6)A modificationalternative polyadenylationnitrate metabolismCPSF30N-methyladenosine (mA), a ubiquitous internal modification of eukaryotic mRNAs, plays a ...
However, there is little evidence documenting the role of m6A in regulating alternative polyadenylation (APA) in plants. APA is controlled by a large protein-RNA complex with many components, including CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR30 (CPSF30). In Arabidopsis, CPSF30 has two ...
2021年1月27日,Molecular Plant在线发表了南方科技大学李思思课题组、中国科学院遗传与发育生物学研究所曹晓风课题组和山东农业大学王勇课题组合作完成的研究论文“CPSF30-L-mediated recognition of mRNAm6A modification controls alternative polyadenylation of nitrate signaling-related gene trans in Arabidopsis”。 m6A...