CPSF30-L是一个特殊的假定m6A结合蛋白,然而,迄今为止仅报道了CPSF30-L在硝酸盐信号传导中发挥作用,CPSF30-L是否为m6A结合蛋白以及是否依赖m6A发挥生物功能及基因调控机制尚无报道。 近日,北京大学化学与分子工程学院贾桂芳课题组在Molecular Plant(IF 12.084)上发表了题为Arabidopsis N6-methyladenosine reader CPSF30-L...
该研究首先利用体外生化实验证明了CPSF30-L的YTH结构域可以优先结合带有m6A修饰的RNA,同时解析了CPSF30-L的YTH结构域结合m6A RNA的复合物结构,获得了参与m6A修饰识别的关键氨基酸位点信息(如:W259,W310和Y319);对带有m6A修饰的基因与poly(A)位点的选择受到CPSF30调控的基因关联分析,发现存在m6A修饰的基因在CPSF30突...
Arabidopsis CPSF30 has two isoforms and the longer isoform (CPSF30-L) contains a YT512-B Homology domain, which is unique to plants. In this study, we showed that the Arabidopsis CPSF30-L YTH domain binds to mA in vitro. In the cpsf30-2 mutant, many genes including several important ...
APA is controlled by a large protein-RNA complex with many components, including CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR30 (CPSF30). In Arabidopsis, CPSF30 has two isoforms and the longer isoform (CPSF30-L) contains a YT512-B Homology (YTH) domain, which is unique to plants. In ...
2021年1月27日,Molecular Plant在线发表了南方科技大学李思思课题组、中国科学院遗传与发育生物学研究所曹晓风课题组和山东农业大学王勇课题组合作完成的研究论文“CPSF30-L-mediated recognition of mRNAm6A modification controls alternative polyadenylation of nitrate signaling-related gene trans in Arabidopsis”。
Li, Z.; Wang, R.; Gao, Y.; Wang, C.; Zhao, L.; Xu, N.; Chen, K.E.; Qi, S.; Zhang, M.; Tsay, Y.F. The Arabidopsis CPSF30-l gene plays an essential role in nitrate signaling and regulates the nitrate transceptor gene NRT1. 1. New Phytol. 2017, 216, 1205-1222. [...
We found the mA binding activity enhances the formation of liquid-like CPSF30-L nuclear bodies, where CPSF30-L mainly recognizes mA-modified far upstream element (FUE) to control polyadenylation site choice. Disruption of CPSF30-L lengthens 3' untranslated region (3' UTR) of three CPSF30-L ...