Sign in to download full-size image FIGURE 60.3.Transcriptional repression by MeCP2. MeCP2 selectively binds 5-methyl cytosine of the CpG dinucleotides through its methyl-CpG-binding domain (MBD); MeCP2 is able to bind to a single methylated CpG pair. The transcriptional repression domain (TR...
we selected one HGE to study in vivo, which has both an oCGI and prior evidence of increased regulatory activity in human compared to mouse. We generated a humanized mouse line for this locus and found that the oCGI-containing human enhancer exhibits increased ...
Sign in to download full-size image Fig. 1.Structures of the human MBD1 protein and the EGFP-MBD-NLS probe. (A) Domain structures of human MBD1. MBD, NLS, three CXXC domains and TRD. (B) Comparison of deduced amino acid sequences of human (upper) and mouse (lower) MBD1. (...
CpG islands, which are clusters of CpG dinucleotides in GC-rich regions, are considered gene markers and represent an important feature of mammalian genomes. Previous studies of CpG islands have largely been on specific loci or within one genome. To date
Full size image Regulatory role of Sp1 on Podxl promoter activity The fragments up to the first 364 bp show a progressive increasing number of Sp1 sites, suggesting their role in transcription. To test this possibility we transfected some of the constructs into the Sp1-deficient Drosophila-derived...
Recent studies have boosted our understanding of long noncoding RNAs (lncRNAs) in numerous biological processes, but few have examined their roles in somatic cell reprogramming. Through expression profiling and functional screening, we have identified th
Full size image PGRMC1 phosphorylation status regulates the NNMT/1-MNA pathway To further characterize metabolic differences between cells (Fig. 1) we performed a pilot metabolomics study, where we observed elevated levels of 1-methylnicotinamide (1-MNA) in some but not all experiments (not shown...
Full size image Using ESC chromatin marks to define PRC2+and PRC2−gene classes has been a common practice in the definition of CpG Island Methylator Phenotype (CIMP) and other cancer methylation signatures, due to the more diffuse distribution of H3K27me3 ChIP-Seq in differentiated cell typ...
Meng, H., Murrelle, E.L., Li, G.: Identification of a small optimal subset of CpG sites as bio-markers from high-throughput DNA methylation profiles. BMC Bioin- form. 9(1), 457 (2008)Meng H, Murrelle EL, Li G. Identification of a small optimal subset of CpG sites as bio-...
The present invention generally concerns the fields of molecular biology and cellular biology. In particular, the present invention regards selection of molecules with a specific base composition, preparation of molecules for a library, and amplification of selected molecules, such as for amplification of...