If this option is provided, TopHat will first extract the transcript sequences and use Bowtie to align reads to this virtual transcriptome first. Only the reads that do not fully map to the transcriptome will t
is the name of any of the index files up to but not including the first period. Bowtie ...
改为/home/u20111230014/miniconda3/envs/python27/bin/python2 后保存 PS: 路径必须要带上/bin/python2 tophat2 -h测试即可! 另一种情况: 报错:Error:CouldnotfindBowtie2index files(genome.*.bt2)或Warning:CouldnotfindFASTAfile bowtie2_index/GRCm39_index.fa但不提示error,照常preparing reads,但生成...
Description I wish to use the latest NanoSim v3.0.1 (supports reading .gz sequence files, and bam files), but read_analysis.py does not complete. The primary.bam file is not indexed, an that might be an issue for pysam, but there seem to...