SNP genotypingKASPAgarose-MAMA assayFusarium wiltresistanceProtocol for 'Mismatch Amplification Mutation Assay' (MAMA) was standardizedAgarose-MAMA assays for three SNP loci linked to wilt resistance in castor were developedThe results of MAMA assays were comparable with KASP assaysAgarose-MAMA assays ...
1. Birdsell DN, Pearson T, Price EP, Hornstra HM, Nera RD, Stone N, Gruendike J, Kaufman EL, Pettus AH, Hurbon AN et al: Melt analysis of mismatch amplification mutation assays (Melt-MAMA): a functional study of a cost-effective SNP genotyping assay in bacterial models. PloS ...
In addition to these, the cost of genotyping using SNP arrays, even after development, is considerably higher than sequencing-based approaches [15]. While genotyping based on whole-genome sequencing (WGS) remains expensive and sometimes unnecessary in the context of large-scale studies, low- (<...
Simple, cost-effective chromatin immunoprecipitation sequencing (ChIP-Seq) DNA library preparation, with master mixes and robust multiplex capabilities.
High throughput human genotyping for variants associated with malarial disease outcomes using custom targeted amplicon sequencing ArticleOpen access26 July 2023 Introduction The malaria parasite speciesPlasmodium falciparumis an example of both the potential value of genomic surveillance and the obstacles that...
Results of the demonstrates the utility of Cicer EST resources and the availability of bioinformatics analysis pipelines for the large-scale identification of SNPs on the HPC (High Performance Computer) at ICRISAT and the development of costeffective CAPS assay for SNP genotyping. It is anticipated ...
More recent studies [90, 91] have reported that probabilistic SNP genotyping of low DNA concentra- tions is feasible. Likely software will be developed in the near future from several sources to facilitate mixture interpretation. It seems reasonable to expect that a large SNP panel may be ...
SNP genotyping or somatic mutation detection has been hampered by its low discriminating power and high costs. To tackle this, we developed a cost-effective qPCR based method, able to detect SNPs in a robust and specific manner. This study describes how to combine the basic principles of ...
We report the development of a microarray platform for rapid and cost-effective genetic mapping, and its evaluation using rice as a model. In contrast to methods employing whole-genome tiling microarrays for genotyping, our method is based on low-cost spotted microarray production, focusing only on...
Concordance between blood- and feces-derived genotyping data from the same individuals. Colors symbolize the six captive individuals included in our study. Within these individuals, we did not find significant differences in (A) the proportion of unique alleles or (B) inbreeding coefficients from blo...