label-free detectionin situ detectionoblique-incidence reflectivity differenceOI-RDimmobilization strategiesWe describe a new oblique-incidence reflectivity difference (OI-RD) scanning microscope for high-throughput screening, in microarray format on functionalized glass slides, small-molecule compound libraries ...
After staining, the slides were imaged using an optical microscope. RNA-seq. The total RNA was extracted from compound 331 treated cells and control cells using the RNeasy mini Kit (Qiagen). The sample was then run using a fragmented RNA on a RNAPico 6000 chip in an Agilent 2100 ...
Micro- scopy analyses were performed with a confocal laser scanning microscope (Leica TCS-SP8 Microscope, Benshein, Germany) equipment with a  63 objective (Plan-Neofluar, 1.4 numerical aperture) under oil immersion using LAS-AF software (Leica). The pinhole device was adjusted to capture ...
Malignant gliomas are the most common malignant tumors in the central nervous system (CNS). Up to date, the prognosis of glioma is still very poor, effective therapy with less side-effect is very necessary. Herein, we identify a compound named as “331”
Silicate Glass The main components of silicate glasses tend to be SiO2-Al2O3-R2O, SiO2-P2O5-R2O, SiO2-B2O3-R2O, SiO2-GeO2-R2O, or a mix of the above, with a high SiO2 content and relatively a low R2O content. The glass often exhibits a small expansion coefficient, small ...
After 70 h of incubation, the plates were inspected under an inverted microscope to assure growth of the controls and sterile conditions. Then, 0.01 mL resazurin solution (resazurin, 12.5 mg in 100 mL double-distilled water) was added to each well and the plates were incubated for another 2...
(#S3023; Dako, Santa Clara, CA, USA). All of the wells were observed with a Leica DMi8 microscope and digital images were captured within the same session from 10 random non-overlapping fields/well using a 40× objective. The IF staining intensities of each targeted protein were measured ...
Slides for coverslips and chamber slides were examined using a FLoid™ Cell Imaging Station (Thermo Fisher Scientific, Waltham, MA, USA) or confocal microscope (Leica TCS SP5, Mannheim, Germany). 4.9. FACS Analyses The expression of antigens on cells untreated or treated with different doses ...
Morphology and Maximum Pore Size Morphology studies of PVDF HFMs were carried out using a scanning electron microscope (Hitachi S-4800, HITACHI, Tokyo, Japan). The membranes were immersed in liquid nitrogen and fractured carefully. The specimens were put on a metal support and dried under vacuum...