AssayCaptoprilCompetitive inhibitor bindingWe describe a new principle for measuring concentrations of pharmacologically active captopril or other angiotensin-converting enzyme (ACE) inhibitors in blood. Serum is incubated with 125I-labelled ACE inhibitor (351A, a p-hydroxy-benzamidine derivative of N-(1...
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A variant of a fluorescence quenching affinity assay is described that is based on intermolecular complexation due to specific interaction between an unmodified multivalent lectin and fluorochrome-labeled dextrans bearing specific sugar ligands (analyte-analog). The measuring principle relies on the fact ...
Define Competitive inhibitor. Competitive inhibitor synonyms, Competitive inhibitor pronunciation, Competitive inhibitor translation, English dictionary definition of Competitive inhibitor. also in·hib·it·er n. One that inhibits, as a substance that r
3. Biochemistry Relating to the inhibition of enzyme activity that results from an alternate compound binding reversibly to the site on an enzyme where the substrate would normally bind. com·pet′i·tive·ly adv. com·pet′i·tive·ness n. American Heritage® Dictionary of the English Languag...
The immunochemical detection principle is based on the competition between a fixed amount of labelled 4-nitrophenol derivative (tracer) and the analyte for a limited amount of antibody binding sites. The separation of free and bound tracer fractions is performed in a restricted-access (RA) column ...
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A thyroxine immunoassay was developed based on the principle of a liquid-phase binding assay. Thyroxine and peroxidase-(POD-) labeled anti-thyroxine monoclonal antibody (Fab'-POD) solutions are mixed, incubated, and analyzed directly by high-performance liquid chromatography using a cation-exchange co...
FIGURE 1. The “antibody binding site occupancy principle” of immunoassay. All immunoassays implicitly rely on the measurement of the fractional antibody binding site occupancy by analyte. Sign in to download full-size image FIGURE 2. Immunoassays differ in the way fractional occupancy of antibody ...
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