Domain Binding and Function Coiled-coils (CC) function as oligomerization domains for a wide variety of proteins including structural proteins, motor proteins and transcription factors. The coiled-coil structure is conserved from viruses to plants and mammals and it has been predicted that approximately...
Fusion of the CC domain to the E3 ubiquitin ligase CHIP/STUB1 renders the latter capable of NF-B activation. Moreover, the CC domain, after oligomerization, interacts with Ubc13/Ub~Ubc13, which further contributes to TRAF6 processivity. Point mutations within the CC domain that weaken TRAF6 ...
CC Christian Church CC Clinical Center (NIH) CC Community Center CC Cruise Control CC City Council CC College Center CC Competition Commission CC Customer Care CC Clear Channel CC Commercial (Top-Level Domain introduced in 2000) CC Coupe Cabriolet (Peugeot 206 CC model) CC Computer Center CC Co...
真核生物中,大部分蛋白呈现出由多个domain和motif构造而成的模块化构造方式(cassette-like fashion).研究表明,蛋白质之间专一性的交互作用也往往由这些domain/motif介导的.Coiled coil就是其中一个介导蛋白质专一性相互作用的domain. 为了系统地了解coiled coil之间专一性的相互作用,我们发展了计算和实验相结合的多步骤的...
具体的筛选流程和结果记录方式如图2.3,蓝色方框 表示方形培养基,白点表示酵母菌落,记录最终四缺陷平板上的茵落生长状况来 确定coiledcoil domain间的相互作用。 AD-CCl 脚酗静int03S4wen Print onto YPDA plaW + AD-CCs(40-·5嘲。搬黝 BK-Ccln搬卜如Inl恕渤 Matingfor48hr Re#lcaw .露娑露尘 ‘翟’ -...
Here, we determine the crystal structure of Brat-CC domain (aa 376-511) at 2.5 , showing that Brat-CC forms an elongated antiparallel dimer through an unconventional CC structure. The dimeric assembly in Brat-CC structure is similar to its counterparts in other TRIM proteins, but Brat-CC ...
(CC) domain at the N terminus of the protein. Here, we describe a rationally engineered mutant version of the CC domain, called CCmut3, which interferes with BCR-ABL1 oligomerization and promotes apoptosis in BCR-ABL1-expressing cells, regardless of kinase domain mutation status. CCmut3exhibits...
When masked using the CC2B domain, a decrease in binding affinity to the target antigen of about 1–3 orders of magnitude was observed for all mAbs using flow cytometry of antigen-positive cells. Antibody activity could be restored relatively close to the original affinities for all antibodies ...
(A))显示,CC1形成一个三聚化卷曲结构,能够将SUN结构域从CC2的抑制作用中释放出来,并促使其形成具有活性的三聚体.在细胞中,CC1和CC2作为SUN2蛋白自身调控因子,分别介导SUN结构域的激活和抑制,协同发挥作用以调控SUN-KASH复合物的形成.结合生化实验,结构解析和细胞功能实验,本文阐述了CC结构域调控SUN结构域活性的...
As such it is highly probable that this protein binds to starch under both day and night conditions [16], and functions as a scaffolding protein for other proteins to bind to via the CC domain. Not all starch binding proteins bind starch tightly all the time. The binding of the dual-...