coiled-coil domaindisease resistanceNLRstructurePlant nucleotide-binding, leucine-rich repeat receptors (NLRs) perceive pathogen avirulence effectors and activate defense responses. Nucleotide-binding, leucine-rich repeat re-ceptors are classified into coiled-coil (CC)-containing and Toll/interleukin-1 ...
的domain。 为了系统地了解coiledcoil之间专一性的相互作用,我们发展了计算和实验 相结合的多步骤的方法。第一步,从酵母(saccharomycescerevisiae)蛋白组中识别 含coiledcoil的蛋白,称为coiled-coil蛋白;第二步,从己知的酵母蛋白质相互作 用网络中抽提一个coiled-coil蛋白间交互作用网络;第三步,以该网络为基础用 SVM...
Coiled coil Drug delivery Peptide Self-assembly Therapeutic Folding Dimerization Conjugation Nanostructure Abbreviations Ala Alanine APNP Activatable protein nanoparticle Asn Asparagine bFGF Basic fibroblast growth factor CBD Collagen-binding-domain CC Cytochrome C CD Circular dichroism CCPO Coiled coil protein ...
Coiled-Coil Domain-Dependent Homodimerization of Intracellular Barley Immune Receptors Defines a Minimal Functional Module for Triggering Cell Death consisting of N-terminal coiled-coil (CC) or TOLL/interleukin-1 receptor (TIR) domains, a central nucleotide-binding (NB) domain, and C-terminal leucin...
真核生物中,大部分蛋白呈现出由多个domain和motif构造而成的模块化构造方式(cassette-like fashion).研究表明,蛋白质之间专一性的交互作用也往往由这些domain/motif介导的.Coiled coil就是其中一个介导蛋白质专一性相互作用的domain. 为了系统地了解coiled coil之间专一性的相互作用,我们发展了计算和实验相结合的多步骤的...
cc4 is derived from the pore-forming toxin YaxAB36 and cc5 represents the microtubule-binding domain from dynein37. Covalent connection of helices by disulfide bonds at their N- or C-terminus is a common approach to stabilize coiled-coil structures35,38. Because some of the isolated coiled-...
Previously, we compared the phosphoproteomes of breast cancer stem cells (BCSCs) enriched subpopulation and non-BCSCs sorted from breast cancer patient-derived xenograft (PDX), and identified a function unknown protein, transmembrane and coiled-coil domain family 3 (TMCC3) to be a potential ...
2.1.2 coiled coil序列的克隆 通过PCR从酵母基因组中扩增预测得到的coiled coil domain的DNA序列, 经合适的酶切,连接到两个双杂载体中。 载体: pGADT7-Ree:E.coli Seleetable Markers:Ampr,Yeast Selectable Markers:LEU2. pGBKT7:E.coli Selectable Markers:Kanr,Yeast Selectable Markers:TRPl. pGADT7.RecT:...
Coiled-coil protein domain analysis The amino acid sequence of At5g39790 and its homologues were tested for coiled-coil (CC) domains by several methods. PairCoil2 ([34]; http://groups.csail.mit.edu/cb/paircoil2/paircoil2.html) and PCOILS ([35]; http://toolkit.tuebingen.mpg.de/pcoils...
Fig. 2. Overall crystal structure of the TRIM75 coiled-coil domain. (A) Schematic representation of TRIM75 domain organization. B2, B box 2; CC, the coiled-coil domain. The residue boundaries of the domains are indicated. The coiled-coil domain is highlighted in green. (B, C) Cartoon ...