F. et al. AID produces DNA double-strand breaks in non-Ig genes and mature B cell lymphomas with reciprocal chromosome translocations. Mol. Cell 36, 631–641 (2009). Article CAS Google Scholar Fujimoto, A. et al. Whole-genome mutational landscape and characterization of noncoding and ...
antisense strand是指反义链 在基因的DNA双链中,转录时作为mRNA合成模板的那条 DNA单链叫做模板链或反义链. 还有的两个就不知道了. 分析总结。 antisensestrand是指反义链在基因的dna双链中转录时作为mrna合成模板的那条dna单链叫做模板链或反义链结果...
and a signature composed of them can be utilized for discriminating the non-survivors from survivors with an AUC of 0.8. Among the above host miRNAs, some of them (e.g., let-7g-tp, miR-363-5p, and miR-4510) can target the SARS-CoV-2 genome, and ...
Damage-induced long noncoding RNA (dilncRNA)Double-strand break (DSB)In vitro transcriptionPlasmid DNADNA damage response (DDR) is essential for the maintenance of genomic integrity. We have recently discovered the generation of noncoding RNA from a DNA double-strand break (DSB) in an MRE11-...
potassium voltage-gated channel, KQT-like subfamily, member 1 opposite strand 1 LC3B-II/MAP1LC3B microtubule-associated protein 1 light chain 3 beta-II LincRNA long intervening noncoding RNA LINCMD1 long noncoding RNA, muscle differentiation MALAT1 metastasis associated lung adenocarcinoma transcript ...
Long non-coding RNAs control hematopoietic stem cell function. Cell Stem Cell. 2015;16(4):426–38. Article CAS PubMed PubMed Central Google Scholar Cabili MN, Trapnell C, Goff L, Koziol M, Tazon-Vega B, Regev A, et al. Integrative annotation of human large intergenic noncoding RNAs ...
Direct RNA-DNA interactions occur through complementary base pairing of DNA with RNA, resulting in the formation of a three-stranded structure consisting of a DNA:RNA hybrid and the displaced complementary DNA strand (Drolet et al.1995; Thomas et al.1976). Tiny, three-stranded “bubbles”occur...
The strand attaches to Argonaute 2 (AGO2), which is the major protein of the RNA-induced silencing complex (RISC). miRNAs are included in the RISC. They can regulate gene expression in a posttranscriptional manner through formation of miRNA response elements (MREs) by binding to specific ...
5a, b). To control for the non-specific fitness effects of double-strand breaks (DSBs)53,54, we also created two neutral control sgRNAs targeting AAVS1 locus, and an efficient positive-control-paired sgRNA (pgRNA) to delete the entire NEAT1_1 region (“KO-sgRNA” in Fig. 5b and ...
Total RNA was diluted to 0.3 ng/μl and reverse transcribed into cDNA using the SuperScript first-strand synthesis system (Invitrogen). Then, 3 ng of cDNA was amplified in a C1000 duel thermal cycler (Bio-Rad) according to the KAPA 2 G Fast PCR Kit protocol (KAPA Biosystems) an...