To identify the mutation, we then sequenced the entire versican coding region, as well as the 5′ and 3′UTRs (untranslated regions) of the lht mutant, and compared the mutant and WT sequences. We found a guanine (G) to thymine (T) transversion (Fig. 2B, arrow) and a thymine inser...
Zygotic genome activation (ZGA) is a critical postfertilization step that promotes totipotency and allows different cell fates to emerge in the developing embryo. MERVL (murine endogenous retrovirus-L) is transiently upregulated at the two-cell stage dur
If you want for some reason to generate the bam files with STAR, use the option --outSAMstrandField intronMotif of STAR to produce files that are compatible wiht StringTie in BRAKER3. In order to run BRAKER with RNA-Seq spliced alignment information that has already been extracted, run: ...
4、pangene['pandʒi:n]：胚芽，泛子5、pangenesis[pæn'dʒenisis]n.[遗]泛生论泛生论（theoryofpangenesis）为达尔文晚年所提出的一个遗传学理论。认为生物体各部分的细胞都带有特定的自身繁殖的粒子，称为“微芽”或“泛子”。这种粒子可由各系统集中于生殖细胞，传递给子代，使它们呈现亲代的特征。...
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Footprinting of the qacAcoding strand utilized the 137-bp DNA fragment used in gel-mobility shift experiments, generated using primers 738 (end-labeled with [γ-32P]ATP) and 867HindIII. For footprinting of the qacA non-coding strand, a 154-bp fragment (bp 738–899; Fig. 1) was prepare...
Two hundred nanograms of fragmented mRNA was reverse-transcribed to first strand cDNA by random priming, using 3 μg of random hexamer oligos and 200 units of Superscript II reverse transcriptase (Invitrogen). The reaction was carried out at 45°C for 1 hr in First Strand Buffer (...
Table S1. RPB1 Ubiquitylation Sites Found in Mass Spectrometry Studies, Related to Figure 1 Table S2. Proteome-wide Ubiquitylation Analysis at Different Time Points after UV Irradiation, Related to Figure 3 Table S3. Parameters Used for RNAPII Activity Simulation, Related to Figures 3 and 4 Table...
Teleost fishes exhibit complex sexual characteristics in response to androgens, such as fin enlargement and courtship display. However, the molecular mechanisms underlying their evolutionary acquisition remain largely unknown. To address this question, w
DNA:DNA:RNA triplexes that are formed through Hoogsteen base-pairing of the RNA in the major groove of the DNA duplex have been observed in vitro, but the extent to which these interactions occur in cells and how they impact cellular functions remains elusive. Using a combination of bioinforma...