Co-IP 流程 (以内源性 Co-IP 为例)[1]1样本准备采用适当的方法收集细胞，使用含有合适的蛋白酶抑制剂的裂解缓冲液裂解细胞。2固相介质准备将含有保护液的蛋白 A/G 磁珠或蛋白 A/G 琼脂糖混匀取出，用平衡液清洗磁珠/琼脂糖凝胶。3预清洗 (可选)将细胞裂解液或已预处理的样本用蛋白 A/G 磁珠或蛋白 A/...
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五、RNA结合蛋白免疫沉淀技术 RNA结合蛋白免疫沉淀技术(RNA Binding Protein ImmunoprecipitationAssay,RIP)主要是用来研究体内、RNA与蛋白结合情况,利用针对目标蛋白的抗体把相应的RNA-蛋白复合物沉淀下来,通过分离纯化,对结合在复合物上的RNA 进行RT-PCR验证或测序分析。 六、RNA pull down实验 顾名思义,是研究RNA与蛋...
(e.g., cell lysate or serum) via its affinity to a specially prepared solid support. It is often possible to detect macromolecules that form stable complexes with the target protein in a pull-down assay—when the pull-down is accomplished us...
[2] Tan L, Yammani RR. Co-Immunoprecipitation-Blotting: Analysis of Protein-Protein Interactions. Methods Mol Biol. 2022;2413:145-154.[3] Lee C. Coimmunoprecipitation assay. Methods Mol Biol. 2007;362:401-6.[4] Tang Z, et al. Analysis of Protein-Protein Interaction by Co-IP in Human...
[5] Husain, Afzal, et al. "Native Co-immunoprecipitation assay to identify interacting partners of chromatin-associated proteins in mammalian cells." Bio-protocol 10.23 (2020): e3837-e3837. [6] Takahashi Y. Co-immunoprecipitation from transfected cells[J]. Protein-Protein Interactions: Methods an...
五、RNA结合蛋白免疫沉淀技术 RNA结合蛋白免疫沉淀技术（RNA Binding Protein ImmunoprecipitationAssay，RIP）...
Here, we describe a simple protocol for performing this co-IP assay to get a measurement of the steady-state levels of agonist-elicited GPCR–arrestin interaction in cells. Introduction Aldosterone is a mineralocorticoid hormone with several cardio-toxic actions, whose plasma levels are extremely ...
[5] Husain, Afzal, et al. "Native Co-immunoprecipitation assay to identify interacting partners of chromatin-associated proteins in mammalian cells." Bio-protocol 10.23 (2020): e3837-e3837. [6] Takahashi Y. Co-immunoprecipitation from transfected cells[J]. Protein-Protein Interactions: Methods an...
[3] Lee C. Coimmunoprecipitation assay. Methods Mol Biol. 2007;362:401-6. [4] Tang Z, et al. Analysis of Protein-Protein Interaction by Co-IP in Human Cells. Methods Mol Biol. 2018;1794:289-296. [5] Burckhardt CJ, Minna JD, Danuser G. Co-immunoprecipitation and semi-quantitative ...