网络免疫共沉淀 网络释义 1. 免疫共沉淀 2.5.7免疫共沉淀(Co-IP assay)64-65 cdmd.cnki.com.cn|基于 1 个网页
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[4] Kuzmanov U, Emili A. Protein-protein interaction networks: probing disease mechanisms using model systems[J]. Genome medicine, 2013, 5: 1-12. [5] Husain, Afzal, et al. "Native Co-immunoprecipitation assay to identify interacting partners of chromatin-associated proteins in mammalian cells....
[4] Kuzmanov U, Emili A. Protein-protein interaction networks: probing disease mechanisms using model systems[J]. Genome medicine, 2013, 5: 1-12. [5] Husain, Afzal, et al. "Native Co-immunoprecipitation assay to identify interacting partners of chromatin-associated proteins in mammalian cells....
RNA结合蛋白免疫沉淀技术(RNA Binding Protein ImmunoprecipitationAssay,RIP)主要是用来研究体内、RNA与蛋白结合情况,利用针对目标蛋白的抗体把相应的RNA-蛋白复合物沉淀下来,通过分离纯化,对结合在复合物上的RNA 进行RT-PCR验证或测序分析。 六、RNA pull down实验 ...
Pull-down 技术又称蛋白质体外结合实验(Binding assay in vitro), 是一种行之有效的验证酵母双杂交系统的体外试验技术,可用于初步研究两种蛋白质互作分析。Co-IP和Pull-down 均可用于检测蛋白相互作用,两者有很多相似之处,包括均能检测两种蛋白质之间强相互作用,但均只能进行定性分析而不能检测瞬时相互作用,...
[5] Husain, Afzal, et al. "Native Co-immunoprecipitation assay to identify interacting partners of chromatin-associated proteins in mammalian cells." Bio-protocol 10.23 (2020): e3837-e3837. [6] Takahashi Y. Co-immunoprecipitation from transfected cells[J]. Protein-Protein Interactions: Methods an...
8. In situ detection of individual mRNA molecules and protein complexes or post-translational modifications using padlock probes combined with the in situ proximity ligation assay, Nat Protoc 8 (2013) 355-372. 参考文献 [1] Mellberg S, Dimberg A, Bahram F, et al. Transcriptional profiling reve...
(e.g., cell lysate or serum) via its affinity to a specially prepared solid support. It is often possible to detect macromolecules that form stable complexes with the target protein in a pull-down assay—when the pull-down is accomplishe...
[5] Husain, Afzal, et al. "Native Co-immunoprecipitation assay to identify interacting partners of chromatin-associated proteins in mammalian cells." Bio-protocol 10.23 (2020): e3837-e3837. [6] Takahashi Y. Co-immunoprecipitation from transfected cells[J]. Protein-Protein Interactions: Methods an...