Cisbio HTRF IP-One assay A second messenger HTRF® assay for Inositol 1-phosphate quantification of GPCR activation
(A,B) Gi signaling for docking hits against human a2AAR in the IP-One assay. 科学家为什么选择HTRF IP-ONE检测? Gq通路是G蛋白介导的信号传导通路之一,其效应分子为磷脂酶C(PLC),当细胞外的信号分子与Gq受体结合后,激活细胞膜上的PLC,水解磷脂酰肌醇4,5-二磷酸(PIP2)为肌醇1,4,5-三磷酸(IP3)和...
Terasawa, YukoZhang, ShengwenVenkat, RadhikaMancebo, Helena
Fig. 1: Cisbio IP-One HTRF assay. Materials & Methods CHO cells stably expressing GalR2 (GE Healthcare) Fresh and Frozen CHO-M1 Cell lines, CCS Culture Service, Germany IP-One HTRF® assay kit (Cisbio) White 384-well small volume plates (Greiner) Single cell clone screeningCHO-K1 ...
IP-Oneassays IP-OneisauniqueassaytoinvestigateGqProtein-Coupled Receptors.IP-Oneassaydetectsinositolmonophosphate, astabledownstreammetaboliteofIP3,inducedbythe activationofphospholipaseC(PLC)cascade.Togetherwith HTRFcAMPassays,IP-Oneprovidesarobustandreliable platformtoassessGPCRtargets,andasuperioralternative tocalc...
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Western及IP细胞裂解液(无抑制剂)(Cell lysis buffer for Western and IP without inhibitors),是一种在非变性条件下裂解细胞或组织样品从而制备蛋白样品的裂解液。本细胞裂解液裂解的细胞或组织样品,可以用于PAGE,Western,免疫沉淀(immunol precipitation,IP),免疫共沉淀(co-IP)和ELISA等,以及许多兼容1% Triton X-...
One Step Cloning Kit (Vazyme, C112-01). The vectors used in the yeast two-hybrid assay were pDBLeu for bait and pPC86 for prey. pSPYCE and pSPYNE vectors were used for the BiFC assay49. The coding regions of targets were inserted into the pGEX-6P-1, pMAL-c2x or pColdTM-TF (...
One advantage of the direct immobilization strategy described above is the lack of Protein A/G as a component, which is a potential source of nonspecific binding interactions in the assay system. The quality of the sample that is used...
Ideal chromatin fragments can range from 200 to >1000 bp; however, DNA shearing is one of the most difficult steps to control. Sonication provides truly randomized fragments, but limitations include the requirement of dedicated machinery...