The chromatin immunoprecipitation (ChIP) assay is a powerful and versatile technique used for probing protein-DNA interactions within the natural chromatin context of the cell (1,2). This assay can be used to i
In this work, a phosphatase reporter assay was used to demonstrate that deletion of Asp1 to eliminate 1,5-IP8 synthesis did not prevent the PHO cascade from responding to Pi depletion. In contrast, it was deletion of the IP6K ortholog kcs1 that blocked the phosphate starvation response (...
Session ID: 2025-06-06:26780be1abeb270e7c4d55e3 Player Element ID: video-9a51631548b2 OK Close Modal Dialog Co-immunoprecipitation (co-IP) Co-immunoprecipitation is an extension of IP that is based on the potential of IP...
Fig. 4. HIF-1α-BNIP3 signaling pathway contributes to mitophagosome formation in renal tubular cells in confocal assay. Representative immunofluorescence images (A) and summarized data (C) showing the inhibitory effect of HIF-1α siRNA on H/R-induced mitophagosome formation as indicated by the...
Given the hypersensitivity of MCM8IP-deficient cells to crosslinking agents, we examined whether MCM8IP deficiency affects replication fork progression upon cisplatin treatment using the DNA fiber assay (Fig. 7a). In untreated conditions, HCT116 cells expressing either control or MCM8IP sgRNAs ...
Quantification of laccase activity and LAC1 gene expression. Laccase activity. The assay for quan- tifying extracellular laccase activity was adapted from23 and is based on the oxidation of the laccase substrate, i323nmμ, dM2Mu′ c-tAeAhdziBaiinmnToSmi-nwbienia)sismf(o3inra-cle6...
The assay was performed according to the manufacturer's instruction. Molecular characterization of microscopy-positive sam- ples and seropositive samples DNA was extracted from microscopy-positive and serology-positive faecal samples using Macherey Nagel NucleoSpin® Soil Kit (MACHEREY-NAGEL GmbH & Co...
Finally, T3 homozygous transgenic and WT seeds were germinated and used in the plant tolerance assays, or the T3 homozygous transgenic seedlings were used in the GUS staining assay. Histochemical GUS staining and expression analysis of GUS under IpLEA-PRO control We subjected the T3 seedlings of ...
The viability of the iP-gp cell monolayers was investigated using the XTT assay. After transepithelial transport of radiolabeled mannitol in the presence of 10 µM of the respective drug candidates, HBSS+ (pH 7.4) was removed followed by addition of 350 µL XTT solution (240 µg/mL and...
(qualitatively) the same enzymatic functionality for substrate conversion. This functionality may be tested by use of an assay system comprising a recombinant yeast cell comprising an expression vector for the expression of the homologue in yeast, said expression vector comprising a heterologous nucleic ...