co-culturegliamicroglianeuronsneuro-inflammationoxidative stressMicroglia, the resident immune cells of the brain, have been implicated in numerous neurodegenerative and neurodevelopmental diseases. Activation of microglia by a variety of stimuli induces the release of factors, including pro- and anti-...
Viviani B. Preparation and coculture of neurons and glial cells. Curr Protoc Cell Biol. 2006; Chapter 2 (Unit 2):7. [ PubMed ]Viviani B (2006) Preparation and coculture of neurons and glial cells. Curr Protoc Cell Biol Chap. 2:Unit 2.7. : 10.1002/0471143030.cb0207s32...
3394ChinMedJ2011;124(20):3394.3398OriginalarticleCo—culturewithmicrogliapromotesneuralstemcellsdifferentiationintoastrocytesGUFeng,WANGJuan,FULiandMAYong-jieKeywords:microglia;stemcells;astrogliogenesisBackgroundNeuralstemcells(NSCs)areaself-renewingandmultipotentpopulationofthecentralnervoussystern(CNS),whichareactivedu...
Cell And Tissue Culture Decapitation Animal Euthanization FACS Cell Proliferation Assay ELISA Reagents Antibodies Protein Experimental Models Rattus norvegicus Kits Other Keywords Microglia Astrocytes Neuroinflammation Citations (18) References Streit WJ, Mrak RE, Griffin WS (2004) Microglia and neuroinflammation...
Figure 1. Schematic model of the glioma tumor microenvironment (TME). The cellular components of the glioma microenvironment include glioma cells and glioma stem cells (GSCs), endothelial cells, pericytes, microglia, macrophages, T cells, neurons, and astrocytes. The figure was created using BioRende...
Consistent with previous reports8,9,12,16,32, SARS-CoV-2 BO infections were detected largely within populations of neurons, neural progenitors and microglia (Extended Data Fig. 3a–d). To test putative virus-induced senescence phenotypes we screened seven SARS-CoV-2 variants by infecting human ...
We also evaluated the effects of rBM-MSC on LPS-stimulated microglia using a reverse coculture system and the same conditions of the transcriptomic analysis. In the transcriptome of rBM-MSCs, 67 genes were differentially expressed, which were highly related with migration of cells, compared to ...
or receptor-mediated endocytosis49,65. Exposure to aggregates stresses iMGLs and iAstrocytes, which then release pro-inflammatory cytokines such as IL-1β, IL-6, TNF-α and chemokine MCP-1, damaging nearby neurons and promoting neurodegeneration66,67. We found that co-aggregation with lipidated...
Oligodendrocytes are the myelin-producing cells of the central nervous system (CNS)1. Myelin, a lipid-rich membrane, insulates the axons of neurons thereby allowing the rapid conduction of electrical impulses and delivery of the action potential to the target cell2,3. Loss of myelin leads to ...
Functional maturation of human neurons has been shown to be improved when co-cultured with rodent astrocytes29,31,32. However, rodent astrocytes significantly differ from human astrocytes33,34 and a co-culture model of human iPSC-derived cortical neurons with human primary astrocytes is currently not...