Cuprizone is toxic for oligodendrocytes, and the model, like the ethidium bromide model of demyelination, partly mimics a form of MS in which oligodendrocyte apoptosis predominates. Remyelination occurs following removal of the toxin from the diet. Experimental autoimmune encephalomyelitis (EAE). An ...
IL-18 is synthesized as an inactive 24-kDa precursor protein that is subsequently processed by caspase-1 into its mature secretable form, which has a molecular weight of 18 kDa [4,12–16]. Pro-IL-18 can also be processed into its active form by various extracellular enzymes including prote...
OurProtocol Optimisationand Clinical Development Plans service is complemented withAdaptive Design competency. Our full-service study/program implementation and execution include: Bioanalytical laboratories to support the development and analysis of biomarker assays in the neurodegenerative disease space ...
motor, and autonomic functions. The most common clinical course involves alternating relapses and remissions where flare-ups of symptoms are followed by periods of full or partial recovery. Common histopathological
Glial cells are abundant in the CNS and are essential for brain development and homeostasis. These cells also regulate tissue recovery after injury and their dysfunction is a possible contributing factor to neurodegenerative and psychiatric disease. Rece
Download: Download full-size image Fig. 1. The zebrafish glut1b promoter drives expression in brain endothelial cells. Confocal microscopy of a live glut1b:mCherry; fli1a:EGFP double transgenic larva at 6 dpf. (A-C) mCherry is spatially restricted to the brain vasculature (B) and overlays...
the Fc region is modified by full or partial deletion of one or more of the CH2region, CH3region, CH4region, and/or hinge region. In some embodiments, the Fc region is modified by full or partial deletion of the C1q binding site. In some embodiments, the Fc region of the antibody is...
Also other cell types including pericytes, astrocytes and neuronal cells in combination with the brain capillary endothelial cells (BCEC), are very important for the full functional ability of the BBB [28], [29], [30]. Compared with brain capillaries in BBB, peripheral capillaries are leaky ...
34 Similarly, since retinal ganglion cells form the last step in the visual processing pathway, expression of therapeutic molecules could be a viable option here also,34 with perhaps more generic retinal disease application. Our efforts have developed at least two MiniPs with bipolar cell expression...
The above techniques are more fully described in laboratory manuals such as “Molecular Cloning: A Laboratory Manual”, Second Edition by Sambrook et al., Cold Spring Harbor Press, 1989; “Current Protocols in Molecular Biology”, Volumes I-III, Ausubel, R. M., ed., 1994; “Cell Biology:...